Abstract
Random amplified polymorphic DNA (RAPD) analysis wasapplied to evaluate variation among provenances of white poplar(Populus alba L.) collected indifferent geographic areas in Italy. Fifteen decamer oligonucleotidesamplified a total of 71 distinct fragments of which 57(80% of the total observed) were polymorphic among53 plants of white poplar. Among provenances, the highest degree ofpolymorphism was detected in the population from Sinni river(62%), while the lowest level was recorded in thepopulation from Arno river (42%). Cluster(UPGMA) analysis was performed on the RAPD data and itdifferentiated the analysed plants in three distinct groupscorresponding to the Apennine, Southern and Northern Italian areas.The RAPD clusters were congruent with groups based on phenologicaltraits.
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References
Bernetti G. 1995. Selvicoltura speciale. UTET, Torino.
Boecklen W.J. and Howard D.J. 1997. Genetic analysis of hybrid zones: Numbers of markers and power of resolution. Ecology 78(8): 2611–2616.
Castiglione S., Wang G., Damiani G., Bandi C., Bisoffi S. and Sala F. 1993. Identification of elite poplar (Populus ssp.) clones using RAPD fingerprints. Theor. Appl. Genet. 87: 60–64.
Dellaporta S.L., Wood J. and Kick J.B. 1983. A plant DNA Minipreparation: Version II. Plant. Mol. Biol. Reporter 1: 19–21.
Demecke T., Adams R.P. and Chibbar R. 1992. Potential taxonomic use of random amplified polymorphic DNA (RAPD): a case study in Brassica. Theor. Appl. Genet. 84: 990–994.
Devos K.M. and Gale M.D. 1992. The use of random amplified polymorphic DNA markers in wheat. Theor. Appl. Genet. 84: 567–572.
Dunn G. and Everitt B.S. 1982. An introduction to mathematical taxonomy. Cambridge University Press, Cambridge, UK.
Grattapaglia D. and Sederoff R. 1994. Genetic linkage maps of Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross: mapping strategy and RAPD markers. Genetics 137: 1121–1137.
Grattapaglia D., Bertolucci F.L. and Sederoff R. 1995. Genetic mapping of QTLs controlling vegetative propagation in Eucalyptus grandis and Eucalyptus urophylla using a pseudo-testcross strategy and RAPD markers. Theor. Appl. Genet. 90: 933–947.
Jaccard P. 1908. Nouvelles recherches sur la distribution florale. Bull. Soc. Vard. Sci. Nat. 44: 223–270.
Kresowich S., Williams J.G.K., McFerson J.R., Routman E.J. and Schaal B.A. 1992. Characterization of genetic identities and relationships of Brassica oleracea L. via a random amplified polymorphic DNA assay. Theor. Appl. Genet. 85: 190–196.
Lapietra G. 1992. Principales caracteristicas de los mas conocidos clones de chopo selecionados en Italia Proc 19th Session of the International Poplar Commission, 22-25 September. Zaragoza, Spain 1:, pp. 542–549.
Legionnet A., Faivre Rampant P., Villar M. and Lefevre F. 1997. Sexual and asexual reproduction in natural stands of Populus nigra. Botanica Acta 110(3): 257–263.
Liu Z.W., Jarret R.L., Duncan R.R. and Kresowich S. 1994. Genetic relationships and variation among ecotypes of seashore paspalum (Paspalum vaginatum) determined by random amplified polymorphic DNA markers. Genome 37: 1011–1017.
Müller-Starck G., Baradat P. and Bergmann F. 1992. Genetic variation within European tree species. New Forests 6: 23–47.
Novy R.G., Kobak C., Goffreda J. and Vorsa N. 1994. RAPDs identify varietal misclassification and regional divergence in cranberry (Vaccinium macrocarpon (Ait.) Pursh.). Theor. Appl. Genet. 88: 1004–1010.
Rohlf F.J. 1993. NTSYS-pc: numerical taxonomy and multivariate analysis system. Exeter Software, Setauket, NY.
Sabatti M. 1994. Ecologia genetica in popolazioni naturali di Populus alba L.: produttivita, fenologia ed efficienza di uso idrico, phd dissertation. University of Tuscia, Viterbo, Italy.
Sanchez N., Grau J.M., Manzanera J.A. and Bueno M.A. 1998. RAPD markers for the identification of Populus species. Silvae Genet. 47(2-3): 67–71.
Sekawin M. 1975. La genetique du Populus alba L. Annales Forèstales, Zagreb 6: 157–189.
Sokal R.R. and Sneath P.H.A. 1973. Numerical taxonomy. The Principles and Practical of Numerical Classification. W. H. Freeman, San Francisco.
Sokal R.R. and Michener C.D. 1958. A statistical method for evaluating systematic relationships. Univ. Kans. Sci. Bull 38: 1409–1438.
Stettler R. 1993. Popla, Molecular Network. 1: 1-2.
Stiles J.I., Lemme C., Sondur S., Morshidi M.B. and Manshardt R. 1993. Using randomly amplified polymorphic DNA for evaluating genetic relationships among papaya cultivars. Theor. Appl. Genet. 85: 697–701.
Tuskan G.A., Francis K.E., Russ S.L., Romme W.H. and Turner M.G. 1966. RAPD markers reveal diversity within and among clonal and seedling stands of aspen in Yellowstone National Park, USA. Can. J. Forest Res. 26(12): 2088–2098.
Williams J.G.K., Kubelik A.R., Livak K.J., Rafalski J.A. and Tingey S.V. 1990. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 18: 6531–6535.
Yeh F.C., Chong D.K.X. and Yang R.C. 1995. RAPD variation within and among natural populations of trembling aspen (Populus tremuloides) from Alberta. J. of Heredity 86(6): 454–460.
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Sabatti, M., D'Ovidio, R., Tanzarella, O.A. et al. Assessment of geographic variation by RAPD markers among Italian open-pollinated progenies of Populus alba L.. Genetic Resources and Crop Evolution 48, 423–428 (2001). https://doi.org/10.1023/A:1012095401517
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DOI: https://doi.org/10.1023/A:1012095401517