Abstract
The RFLP technique has been employed to investigate phylogenetic relationships between species of the Hedysarum genus. Homologous DNA probes were used to generate patterns from a set of the Mediterranean group. The degree of band sharing was used to estimate genetic distances between species and to draw phylogenetic trees. These are in good agreement with phylogeny based on morphological and isozyme markers, but contain novel insights. The results are discussed in the context of current work in molecular biosystematics in Hedysarum complex. Attempts made in this approach to access the extent of variability at the DNA level occurring with the domestication process have been investigated. With the availability of the tested probes, it has been assumed that the genetic structure is not affected by the domestication process.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Amarger V. and Mercier L. 1996. Nuclear ribosomal unit length variation: a putative marker of genetic diversity in jojoba. Int. J. Plant Sci. 153: 296–302.
Baatout H., Marrakchi M., Mathieu C. and Vedel, F. 1985. Variation of plastid and mitochondrial DNA in the genus Hedysarum. Theor. Appl. Genet 70: 577–584.
Baatout H., Marrakchi M. and Pernes J. 1990. Electrophoretic studies of genetic variation in natural populations of allogamous Hedysarum capitatum and autogamous Hedysarum euspinosissimum. Plant Sci. 69: 49–64.
Baatout H., Marrakchi M. and Combes C. 1991. Genetic divergence and allozyme variation within and among populations of Hedysarum spinosissimum sub sp. capitatum and subsp. spinosissimum (Papilionaceae). Taxon 40: 239.
Bonciarelli F. and Monotti M. 1976. 'Grimaldi' una nuova varieta di sulla (Hedysarum coronarium L.). Riv. Agron. 10: 52–56.
Borisjuk N., Borisjuk L., Petjuch G. and Hemleben V. 1993. Comparison of nuclear ribosomal RNA genes among Solanum species and other Solanaceae. Genome 37: 271–279.
Boussaid M., Ben Fadhel N., Trifi-Farah N. and Marrakchi M. 1995. Mediterranean species of Hedysarum genus: genetic resources of forage and grass plants, pp. 115–130, INRA and BRG France.
Chang C. and Meyerowitz E.M. 1991. Plant genome studies: restriction fragment length polymorphism and chromosome mapping information. Curr. Opin. Biotechnol. 1: 423–437.
Chriki A. and Harborne J.B. 1983. Anthocyanins of Hedysarum coronarium and their contribution to flower colour variation. Phytochemistry 22: 2322–2323.
Cordesse F., Cooke R., Tremousaygue D., Grellet F. and Delseny M. 1993. Fine structure and evolution of the rDNA intergenic spacer in rice and other cereals. J. Mol. Evol. 36: 369–379.
Dellaporta S.L., Wood J. and Hicks J.B. 1983. A plant DNA minipreparation: version II. Plant Mol. Biol. Rep. 1: 19–21.
Delseny M., McGrath J.M., This P., Chevre A.M. and Quiros C.F. 1990. Ribosomal RNA genes in diploid and amphidiploid Brassica and related species: organisation, polymorphism, and evolution. Genome 33: 733–744.
Faivre-Rampant P., Jeandroz S., Lefevre F., Lemoine M., Villar M. and Berville A. 1992. Ribosomal DNA studies in poplars: Populus deltoides, P. nigra, P. trichocarpa, P. maximowiczii, and P. alba Genome 35: 733–740.
Graner A. and Wenzel G. 1992. Towards an understanding of the genome. New molecular markers increase the efficiency of plant breeding. Agro-Food Indust. Hi-Tech. 3: 18–23.
Jeandroz S., Pugine A. and Bervillé A. 1996. Cloning and analysis of a 6.8 Kb r-DNA intergenic spacer region of the European ash (Fraxinus excelsior L.). Theor. Appl. Genet. 92: 1003–1008.
Kabbaj A., Zeboudj F., Peltier D., Tagmount A., Tersac M., Dulieu H. and Bervillé A. 1995. Variation and phylogeny of the ribosomal DNA unit types and 5S DNA in Petunia jussieu. Genet. Resourc. Crop Evol. 42: 311–325.
Kim W.K., Innes R.L. and Kerber E.R. 1992. Ribosomal DNA repeat unit polymorphism in six Aegilops species. Genome 35: 510–515.
Le Houerou H.N. 1969. Principes, méthodes et techniques d'amélioration pastorale et fourragère. Pâ turage et cultures fourragères. Etude no. 2. F.A.O. Rome.
Nei M. and Li W. 1979. Mathematical models for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76: 5269–5273.
Nickrent D.L. and Patrick J.A. 1998. The nuclear ribosomal DNA intergenic spacers of wild and cultivated soybean have low variation and cryptic subrepeats. Genome 41: 183–192.
Page R.D.M., 1998. http://taxonomy.Zoology.Gla.ac.uk/Rod/ Rod.html.
Pillay M. and Kenny S.T. 1996. Structure and inheritance of ribosomal DNA variants in cultivated and wild hop, Humulus lupulus L. Theor. Appl. Genet. 93: 333–340.
Polanco C. and Perez De La Vega M. 1994. The structure of the rDNA intergenic spacer of Avena sativa L.: a comparative study. Plant Mol. Biol. 25:75 1–756.
Sambrook J., Fritisch E.F. and Maniatis T. 1989. Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
Santoni S. and Bervillé A. 1992. Characterization of the nuclear ribosomal DNA units and phylogeny of Beta L. wild forms and cultivated beets. Theor. App. Genet. 83: 533–542.
Southern E.M. 1975. Detection of specific sequences among DNA fragments separated by electrophoresis. J. Mol. Biol. 98: 503–518.
Trifi-Farah N., Chatti W.S., Marrakchi M. and Pernes I. 1989. Morphological and enzymatic variability from spontaneous and cultivated forms in Hedysarum coronarium L. Agronomy 9:59 1–598.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Neila, TF., Mohamed, M. Hedysarum phylogeny mediated by RFLP analysis of nuclear ribosomal DNA – RFLP and Hedysarum phylogeny. Genetic Resources and Crop Evolution 48, 339–345 (2001). https://doi.org/10.1023/A:1012051410501
Issue Date:
DOI: https://doi.org/10.1023/A:1012051410501