Abstract
A new reporter-based assay for the evaluation of gap junctional intercellular communication (GJIC) is presented. This assay was applied to the study of endogenous GJIC as well as to the evaluation of cell-to-cell communication exogenously induced in non-coupling cells by transfection with connexin 32. The results obtained with 18-α-glycyrrhetinic acid indicate that this assay system can be used to monitor the GJIC induced by transport of cAMP induced by activation of the dopamine 1 receptor cascade.
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Dearry A,Gingrich A,Falardeau, P,Fremeau R,Bates M,Caron M (1990) Molecular cloning and expression of the gene for a human D1 dopamine receptor. Nature 347: 72-76.
George C,Martin P,Evans W (1998) Rapid determination of gap junction formation using HeLa cells microinjected with cDNAs encoding wild-type and chimeric connexins. Biochem. Biophys. Res. Comm. 247: 785-789.
Goldberg G,Bechberger J,Naus C (1995) A pre-loading method of evaluating gap junctional communication by fluorescent dye transfer. BioTechniques 18: 490-497.
Graeber S,Hulser D (1998) Connexin transfection induces invasive properties in HeLa cells. Exp. Cell Res. 243: 132-149.
Komatsu R,Tsushima N,Matsuyama T (1997) Effects of glucagon administration on microcirculation and blood rheology. Clin. Hemorheol. Micro. 17: 271-277.
Kumar N,Gilula N (1996) The gap junction communication channel. Cell 84: 381-388.
Paul DL (1986) Molecular cloning of cDNA for rat liver gap junction protein. J. Cell Biol. 103: 123-134.
Perez Velazquez J,Carlen P (2000) Gap junctions, synchrony and seizures. Trends Neurol. Sci. 23: 68-74.
Stojkovic T,Latour P,Vandenberghe A,Hurtevent J,Vermersch P (1999) Sensineuronal deafness in X-linked Charcot-Marie-Tooth disease with connexin 32 mutation (R142Q). Neurology 42: 1010-1014.
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Barbier, A.J., Baron, B.M. Development of a prototype reporter-based assay for the evaluation of gap junctional intercellular communication. Biotechnology Letters 23, 1559–1563 (2001). https://doi.org/10.1023/A:1011965209834
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DOI: https://doi.org/10.1023/A:1011965209834