Abstract
We have studied the action of urea and glucose on the stability of DNAand micelles. We measured the melting temperature of aqueous solutionsof DNA with urea or glucose as a co-solute; we have also measured thechanges in the critical micelle concentrations (cmc) of Sodium DodecylSulfate and Triton X-100 by addition of urea and glucose. Ourexperimental results show that glucose increases the melting temperature ofDNA and decreases the cmc, while urea acts in the contrary direction. The effects of urea and glucose on the stability of DNA and micelles canbe explained by the weakening and enhancement of hydrophobicinteractions, respectively. These effects on hydrophobic interactions arediscussed in this paper.
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References
Kautzman, W.: Some Factors in the Interpretation of Protein Denaturation, Adv. Protein Chem. 16 (1959), 1–64.
Re, M., Laría, D. and Fernandez Prini, R.: 'Solvent Structural Contributions to the Dissolution Process of an Apolar Solute in Water, Chem. Phys. Letters 250 (1996), 25–30.
Hallenga, K, Grigera, J.R. and Berendsen, H.J.C.: 'Influence of Hydrophobic Solutes on the Dynamics Behavior of Water, J. Phys. Chem. 84 (1980), 2381–2390.
Matubayasi, N.: 'Matching-Mismatching of Water Geometry and Hydrophobic Hydration, J. Am. Chem. Soc. 116 (1994), 140–1456.
Gekko, K. and Timasheff, S.N.: 'Mechanism of Protein Stabilization by Glycerol: Preferential Hydration in Glycerol-Water Mixtures, Biochemistry 17 (1981), 615–623.
Corrin, A.L. and Harkins, W.D.: 'The effect of Salts on the Critical Concentration for the Formaton of Micelles in Colloidal Electrolytes, J. Am. Chem. Soc. 52 (1947), 130–688.
Schick, M.J. Effect of Electrolyte and Urea on Micelle Formation, J. Phys. Chem. 68 (1964), 3585–3592.
Kresheck, G.C.: Water. A Comprehensive Treatise, Volume 4, Chapter 2. Ed., Felix Franks, Plenum Press, New York and London, 1978.
Nishikido, N. and Matuura, R.: The Effect of Added Inorganic Salts on the Micelle Formation of Nonionic Surfactants in Aqueous Solutions, Bull. Chem. Soc. Japan 50 (1977), 1690–1694.
Malik, W.V. and Saleem, S.M.: 'Effect of Additives on the Critical Micelle Concentration of Some Polyethoxylated Nonionic Surfactants, J. Am. Oil Chem. Soc. 45 (1968), 670–672.
Florenzano, F.H., Cardoso dos Santos, L.G., Cuccovia, I.M, Scarpa, M.V. Chaimovich, H. and Politi, M.J.: Urea-Induced Decrease of Anion Selectiviy In Surfactant Aggregates, Langmuir 12 (1966), 1166–1171.
Walrafen, G.E.: Raman Spectral Studies on the Effect of Urea and Sucrose on Water Structure, J. chem. Phys. 44 (1966), 3726–3727.
Tait M.J., Sugget, A., Franks, F., Ablett, S. and Quickenden, P.R.: Hydration of Monosaccharides. A Study by Dielectrics and Nuclear Magnetic Resonance, J. Solution Chem. 1 (1972), 131–151.
Franks, F. and Grigera, J.R.: Solution Properties of Low Molecular Weight Polyhydroxy Compounds, Water Sci. Rev. 5 (1990), 187–289. (F. Franks, editor, Cambridge University Press, Cambridge).
Galema, S.A., Howard, E.I., Engberts, J.B.F.N. and Grigera, J.R.: The Effect of Stereochemistry upon Carbohydrate Hydration. A Molecular Dynamics Simulation of α-D-Galactopyranose and α-β-Talopyranose, Carbohydrate Res. 265 (1994), 215–225.
Brady, J.W.: Molecular Dynamics Simulations of α-D-Glucose in Aqueous Solution, J. Am. Chem. Soc. 108 (1989), 5155–5165.
Brady, J.W.: Molecular Dynamics Simulations of Carbohydrates Molecules, Adv. Biophys. Chem. 1 (1990), 155–202.
Grigera, J.R.: Hydration of Carbohydrates as Seen by Computer Simulation, Adv. Comp. Biol. 1 (1994), 203–229.
Shelton, V.M., Sosnick, T.R. and Pan, T.: Applicability of Urea in the Thermodynamic Analysis of Secondary and Tertiary RNA Folding, Biochemistry 38 (1999), 16831–16839.
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de Xammar Oro, J.R. Role of Co-Solute in Biomolecular Stability: Glucose, Urea and the Water Structure. Journal of Biological Physics 27, 73–79 (2001). https://doi.org/10.1023/A:1011890506834
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DOI: https://doi.org/10.1023/A:1011890506834