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Expression patterns and subcellular localization of a 52 kDa sucrose-binding protein homologue of Vicia faba (VfSBPL) suggest different functions during development

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Abstract

A cDNA coding for a 54 kDa signal sequence containing protein has been isolated from a faba bean cotyledonary library and characterized. The deduced protein is designated Vicia faba SBP-like protein (VfSBPL) since it shares 58% homology to a 62 kDa soybean (Glycine max) protein (GmSBP) which has been described as a sucrose-binding and sucrose-transporting protein (SBP). VfSBPL as well as GmSBP are outgroup members of the large vicilin storage protein family. We were unable to measure any sucrose transport activity in mutant yeast cells expressing VfSBPL. During seed maturation in late (stage VII) cotyledons mRNA was localized by in situ hybridization in the storage parenchyma cells. At the subcellular level, immunolocalization studies proved VfSBPL accumulation in storage protein vacuoles. However, mRNA localization in stage VI cotyledons during the pre-storage/storage transition phase was untypical for a storage protein in that, in addition to storage parenchyma cell labelling, strong labelling was found over seed coat vascular strands and the embryo epidermal transfer cell layer reminiscent of sucrose transporter localization. The VfSBPL gene is composed of 6 exons and 5 introns with introns located at the same sites as in a Vicia faba 50 kDa vicilin storage protein gene. The time pattern of expression as revealed by northern blotting and the GUS accumulation pattern caused by a VfSBPL-promoter/GUS construct in transgenic tobacco seeds was similar to a seed protein gene with increasing expression during seed maturation. Our data suggest different functions of VfSBPL during seed development.

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Authors and Affiliations

  1. Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), Corrensstrasse 3, 06466 , Gatersleben, Germany

    Ute Heim, Qing Wang, Thorsten Kurz, Ljudmilla Borisjuk, Sabine Golombek, Birgit Neubohn, Klaus Adler, Hans Weber & Ulrich Wobus

  2. Lehrstuhl für Botanik II, Universität Erlangen-Nürnberg, 91058 , Erlangen, Germany

    Manfred Gahrtz & Norbert Sauer

Authors
  1. Ute Heim
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  2. Qing Wang
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  3. Thorsten Kurz
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  4. Ljudmilla Borisjuk
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  5. Sabine Golombek
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  6. Birgit Neubohn
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  7. Klaus Adler
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  8. Manfred Gahrtz
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  9. Norbert Sauer
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  10. Hans Weber
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  11. Ulrich Wobus
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Heim, U., Wang, Q., Kurz, T. et al. Expression patterns and subcellular localization of a 52 kDa sucrose-binding protein homologue of Vicia faba (VfSBPL) suggest different functions during development. Plant Mol Biol 47, 461–474 (2001). https://doi.org/10.1023/A:1011886908619

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