Skip to main content
Log in

Separation of different sized particles by inertial migration

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Inertial migration has been used to fractionate feed streams containing latex particles and sheep leukocytes. For latex particles, a purified stream of small particles, less than 4 μm, was obtained. For sheep leukocytes, a stream containing almost no large leukocytes greater than 16 μm was produced. The results obtained show that this method could be used to fractionate biological cells of different sizes. Inertial migration is most suitable for the removal of larger particles which contaminate a dilute suspension of smaller particles.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Bretherton FP (1962) The motion of rigid particles in shear flow at low Reynolds number. J. Fluid Mech. 14: 284-304.

    Google Scholar 

  • Brugger W,Bross KJ,Glatt M,Weber F,Menelsmann R,Kanz L (1994) Mobilization of tumor cells and hematopoietic progenitor cells in peripheral blood of patients with solid tumors. Blood 83: 636-640.

    Google Scholar 

  • Cox RG,Brenner H (1968) The lateral migration of solid particles in Poiseuille flow-I Theory. Chem. Eng. Sci. 23: 147-173.

    Google Scholar 

  • Elias AD,Ayash L,Anderson KC,Hund M,Wheller C,Schwartz G,Telpler J,Mazanet R,Lynch C,Pap S,Pelsez J,Reich E,Critchelow J,Demetri G,Bibbo J,Schnipper L,Griffin JD,Frei E,Aptman KH (1992) Mobilization of peripheral blood progenitor cells by chemotherapy and granulocyte-macrophage colony-stimulating factor for hematologic support after highdose intensification in breast cancer. Blood 79:3036.

  • Gazitt Y,Reading CC,Hoffman R,Wickrema A,Vesole DH,Jagannath S,Condino J,Lee B,Barlogie B,Tricot G (1995) Purified CD34+ Lin-Thy+ stem cells do not contain clonal myeloma cells. Blood 86: 381-389.

    Google Scholar 

  • Ho BP,Leal LG (1974) Inertial migration of rigid spheres in twodimensional unidirectional flows. J. Fluid Mech. 65, 365-400.

    Google Scholar 

  • Karnis A,Goldsmith HL,Mason SG (1963) Axial migration of particles in Poiseuille flow. Nature 200: 159-160.

    Google Scholar 

  • Kessinger A,Armitage JO (1991) The evolving role of autologous peripheral stem cell transplantation following high-dose chemotherapy for malignancies. Blood 77: 221.

    Google Scholar 

  • Moss TJ,Ross AA (1992) The risk of tumor cell contamination in peripheral blood stem cell collections. J. Hematother. 1: 225-232.

    Google Scholar 

  • Oliver DR (1962) Influence of particle rotation on radial migration in the Poiseuille flow of suspensions. Nature 194: 1269-1271.

    Google Scholar 

  • Poflee NM,Rakow AL,Werntz M,Pons MN (1994) Stagewise design for concentration of Spirulina platensis based on lateral migration with flow through vertical tees. Bioseparation 4: 237-246.

    Google Scholar 

  • Rakow AL,Chappell ML (1987) Axial migration of Spirulina microalgae in laminar tube flow. Biorheology 24: 763-768.

    Google Scholar 

  • Rakow AL,Fernald D (1991) Concentration of Spirulina suspensions by radial migration with flow through vertical tees. Biotechnol. Prog. 7: 343-347.

    Google Scholar 

  • Rakow AL,Chappell ML,Long RL (1989) Concentration of bioparticles by axial migration in laminar flow. Biotechnol. Prog. 5: 105-110.

    Google Scholar 

  • Sanderson JH,Phillips EP (1981) An Atlas of Animal Haematology. Oxford: Clarendon Press.

  • Segré G,Silberberg A (1961) Radial particle displacements in Poiseuille flow of suspensions. Nature 189: 209-210.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to S.R. Wickramasinghe.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wickramasinghe, S., Lin, WC. & Dandy, D. Separation of different sized particles by inertial migration. Biotechnology Letters 23, 1417–1422 (2001). https://doi.org/10.1023/A:1011674015618

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1011674015618

Navigation