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IL-5 Induces Proliferation and Activation of Microglia via an Unknown Receptor

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Abstract

While the effects of interleukin-3 (IL-3) and granulocyte macrophage-colony stimulating factor (GM-CSF) on microglia are well documented, very little is known about the effects of a related cytokine, interleukin-5 (IL-5). We therefore undertook studies to determine how IL-5 alters various aspects of microglial functioning. Treatment of microglia with IL-5 resulted in the induction of proliferation at levels similar to those induced by GM-CSF. IL-5 also increased cellular metabolism of microglial cells. To determine whether increased metabolism correlated with activation of microglia, we measured levels of nitrite, a breakdown product of nitric oxide. Treatment of microglial cultures with IL-5 increased nitrite levels, while GM-CSF treatment had no effect. Treatment of microglia with IL-5 did not cause activation of the signal transduction pathways linked to the classical IL-5 receptor, STAT5A/5B and ERK1 and ERK2. It is therefore likely that the effects of IL-5 on microglia are not mediated via the classical IL-5 receptor, but rather via a novel receptor.

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  1. Departments of Neurobiology and Psychiatry, Mental Retardation Research Center, Brain Research Institute, UCLA School of Medicine, Los Angeles, California

    Stephanie M. Liva & Jean de Vellis

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Liva, S.M., de Vellis, J. IL-5 Induces Proliferation and Activation of Microglia via an Unknown Receptor. Neurochem Res 26, 629–637 (2001). https://doi.org/10.1023/A:1010983119125

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