Abstract
A detailed anaesthetic technique for baboons (Papio anubis) undergoing heterotopic abdominal cardiac xenotransplantation is described. Twenty-two baboons served as transplant recipients. Donors were either crossbred farm pigs (Sus scrofa) (n = 4) or transgenic pigs (Sus scrofa) (n = 18) expressing human complement regulatory proteins on the endothelium. Intra-operative management was complicated by the physiological consequences of infrarenal, abdominal aortic cross-clamping, in addition to the immunological sequelae related to cross-species transplantation. In choosing anaesthetics for this procedure, we considered the need for maximal cardiac stability throughout a long surgical procedure that required abdominal aortic cross-clamping to facilitate the implantation of an oversized porcine cardiac graft. Baboons received a balanced anaesthetic consisting of inhaled isoflurane in oxygen, intravenous fentanyl and intravenous pancuronium. The pharmacological techniques employed were found to be safe and reliable and were well tolerated by our recipients without any significant side-effects.
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REFERENCES
Adams, D.H., Chen, R.H., Kadner, A. and Naficy, S., 1999. Technique for heterotopic pig heart xenotransplantation in primates. Annals of Thoracic Surgery, 68, 265–268
Attia, R.R., Murphy, J.D., Snider, M., Lappas, D.G., Darling, R.C. and Lowenstein, E., 1976. Myocardial ischemia due to infrarenal aortic cross-clamping during aortic surgery in patients with severe coronary artery disease. Circulation, 53, 961–965
Bovill, J.G. and Boer, F., 1993. Opioids in cardiac anesthesia. In: J. Kaplan (ed.), Cardiac Anesthesia, (Saunders, Philadelphia), 495–497
Chen, R.H., Naficy, S., Logan, J.S., Diamond, L.E. and Adams, D.H., 1999. Hearts from transgenic pigs constructed with CD59/DAF genomic clones demonstrate improved survival in primates. Xenotransplantation, 6, 194–200
Cooper, D., Ye, Y., Rolf, J. and Zuhdi, N., 1991. The pig as a potential organ donor for man. In: D. Cooper, E. Kemp, K. Reemtsma and D. White (eds), Xenotransplantation: The Transplantation of Organs and Tissues Between Species, (Berlin, Springer-Verlag), 481
Fridman, E.P. and Popova, V.N., 1988. Species of the genus Papio (Cercopithecidae) as subjects of biomedical research. I. Biological basis of experiments on baboons. Journal of Medical Primatology, 17, 291–307
Gooding, J.M., Archie, J.P. Jr. and McDowell, H., 1980. Hemodynamic response to infrarenal aortic cross-clamping in patients with and without coronary artery disease. Critical Care Medicine, 8, 382–385
Gravlee, G.P., Ramsey, F.M., Roy, R.C., Angert, K.C., Rogers, A.T. and Pauca, A.L., 1988. Rapid administration of a narcotic and neuromuscular blocker: a hemodynamic comparison of fentanyl, sufentanil, pancuronium, and vecuronium. Anesthesia and Analgesia, 67, 39–47
Harrison, G.A., 1972. The cardiovascular effects and some relaxant properties of four relaxants in patients about to undergo cardiac surgery. British Journal of Anaesthesia, 44, 485–494
Hobbhahn, J., Conzen, P., Forst, H. and Peter, K., 1989. Effect of inhalation anesthetics on the myocardium. Anaesthetist, 38supplement 2, S561–596
Institute of Laboratory Animal Resources (US), 1996. Guide for the Care and Use of Laboratory Animals, (National Academy Press, Washington DC)
Maclean, J.M., Phippard, A.F., Thompson, J.F., Gillin, A.G., Horvath, J.S., Duggin, G.G. and Tiller, D.J., 1990. Hemodynamics of conscious unrestrained baboons, including cardiac output. Journal of Applied Physiology, 68, 2373–2379
McCurry, K.R., Parker, W., Cotterell, A.H., Weidner, B.C., Lin, S.S., Daniels, L.J., Holzknecht, Z.E., Byrne, G.W., Diamond, L.E. and Logan, J.S. et al., 1997. Humoral response to pig-to-baboon cardiac transplantation: implications for the pathogenesis and treatment of acute vascular rejection and for accommodation. Humoral Immunology, 58, 91–105
Moffitt, E.A., McIntyre, A.J., Barker, R.A., Imrie, D.D., Murphy, D.A., Landymore, R.W. and Kinley, C.E., 1986. Myocardial metabolism and hemodynamic responses with fentanyl-enflurane anesthesia for coronary arterial surgery. Anesthesia and Analgesia, 65, 46–52
Pagel, P.S., Kampine, J.P., Schmeling, W.T. and Warltier, D.C., 1991. Comparison of the systemic and coronary hemodynamic actions of desflurane, isoflurane, halothane, and enflurane in the chronically instrumented dog. Anesthesiology, 74, 539–551
Popilskis, S.J. and Kohn, D.F., 1997. Anesthesia and analgesia in nonhuman primates. In: D. Kohn, S. Wixson, W. White and G. Benson (eds), Anesthesia and Analgesia in Laboratory Animals, (Academic Press, San Diego), 240
Reiz, S., Peter, T. and Rais, O., 1979. Hemodynamic and cardiometabolic effects of infrarenal aortic and common iliac artery declamping in man — an approach to optimal volume loading. Acta Anaesthesioligica Scandinavica, 23, 579–586
Roizen, M.F., Beaupre, P.N., Alpert, R.A., Kremer, P., Cahalan, M.K., Shiller, N., Sohn, Y.J., Cronnelly, R., Lurz, F.W., Ehrenfeld, W.K. et al., 1984. Monitoring with two-dimensional transesophageal echocardiography. Comparison of myocardial function in patients undergoing supraceliac, suprarenal-infraceliac, or infrarenal aortic occlusion. Journal of Vascular Surgery, 1, 300–305
Slogoff, S., Keats, A.S., Dear, W.E., Abadia, A., Lawyer, J.T., Moulds, J.P. and Williams, T.M., 1991. Steal-prone coronary anatomy and myocardial ischemia associated with four primary anesthetic agents in humans. Anesthesia and Analgesia, 72, 22–27
Stevens, W.C., Cromwell, T.H., Halsey, M.J., Eger, E.I.d., Shakespeare, T.F. and Bahlman, S.H., 1971. The cardiovascular effects of a new inhalation anesthetic, Forane, in human volunteers at constant arterial carbon dioxide tension. Anesthesiology, 35, 8–16
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Santerre, D., Chen, R., Kadner, A. et al. Anaesthetic Management of Baboons Undergoing Heterotopic Porcine Cardiac Xenotransplantation. Vet Res Commun 25, 251–259 (2001). https://doi.org/10.1023/A:1010683907590
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DOI: https://doi.org/10.1023/A:1010683907590