Abstract
An effective protocol has been developed for plant regeneration from cotyledon explants of Swainsona salsula Taubert (Saline swainsona), a medicinal and agronomic shrub. Adventitious shoots were obtained from 83.2% of cotyledon explants from 3-day seedlings cultured on Murashige and Skoog (MS) medium containing 2.0 mg l−1 thidiazuron (TDZ), with an average of 9.3 shoots per explant. Individual elongated shoots were rooted on half strength MS medium supplemented with 2.0 mg l−1 indole-3-butyric acid (IBA), with 59.3% success. Regenerated plants with well developed shoots and roots were successfully transferred to soil, without detectable variants. Histological observation revealed that shoots developed from cotyledon explants via organogenesis, with little callus.
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Yang, J., Hu, Z., Guo, G. et al. In vitro plant regeneration from cotyledon explants of Swainsona salsula Taubert. Plant Cell, Tissue and Organ Culture 66, 35–39 (2001). https://doi.org/10.1023/A:1010679500619
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DOI: https://doi.org/10.1023/A:1010679500619