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Esterase activity assay by flow injection analysis (FIA)

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Abstract

An automatic flow injection analysis (FIA) system for on-line determination of esterase activity has been developed. It is based on a colorimetric method using p-nitrophenyl propionate as substrate. The system permits a linear range analysis up to 0.18 U ml−1, although the range can be extended up to 1 U ml−1 without external dilution of the sample. The sampling frequency is of 4 samples per h with a relative standard deviation of 0.9%.

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Authors and Affiliations

  1. Escola Universitària Politècnica del Medi Ambient, 08100, Mollet del Vallès, Spain

    M.A. Joga, X. Font & M.A. Gordillo

  2. Departament d'Enginyeria Química, E.T.S.E. Universitat Autònoma de Barcelona, 08193, Bellaterra, Spain

    F. Valero

Authors
  1. M.A. Joga
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  2. X. Font
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  3. M.A. Gordillo
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  4. F. Valero
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Joga, M., Font, X., Gordillo, M. et al. Esterase activity assay by flow injection analysis (FIA). Biotechnology Letters 23, 943–948 (2001). https://doi.org/10.1023/A:1010522818725

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  • DOI: https://doi.org/10.1023/A:1010522818725

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