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Improvements in the determination of manganese peroxidase activity

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Abstract

The existing method of determining the activity of manganese peroxidase (MnP), produced by Phanerochaete chrysosporium, was improved. 2,6-Dimethoxyphenol at 80 mM was used as a substrate and, after the decolorization of the reaction mixture, H2O2 was added and the initial reaction rate was used to determine MnP activity.

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Authors and Affiliations

  1. Faculty of Chemistry and Chemical Technology, University of Ljubljana, Aškerčeva 5, 1000, Ljubljana, Slovenia

    I. Grgič, H. Podgornik, M. Berovič & A. Perdih

Authors
  1. I. Grgič
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  2. H. Podgornik
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  3. M. Berovič
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  4. A. Perdih
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Correspondence to I. Grgič.

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Grgič, I., Podgornik, H., Berovič, M. et al. Improvements in the determination of manganese peroxidase activity. Biotechnology Letters 23, 1039–1042 (2001). https://doi.org/10.1023/A:1010508823752

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  • DOI: https://doi.org/10.1023/A:1010508823752

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