Abstract
Kinetic analysis of the reduction of Cr(VI) by resting cell suspensions of Desulfovibrio vulgaris ATCC 29579 and a new isolate, Desulfovibrio sp. (`Oz7') was studied using lactate as the electron donor at 30 °C. The apparent K m (K m app) and V max with respect to Cr(VI) reduction was compared for both strains. Desulfovibio sp. `Oz7' had a K m app of 90 μM (threefold lower than that of D. vulgaris ATCC 29579) and a V max of 120 nmol h−1 mg−1 biomass dry wt (approx. 30% lower than for the reference strain). The potential of the new isolate for bioremediation of Cr(VI) wastewaters is discussed.
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References
Cotton FA, Wilkinson G (1980) Chemistry of the Transition Elements, Advanced Inorganic Chemistry. Chichester: John Wiley & Sons, pp. 719-735.
Cross HJ, Faux SP, Sadhra S, Sorahan T, Levy LS, Aw TC, Braithwaite R, McRoy C, Hamilton L, Calvert I (1997) Criteria Document for Hexavalent Chromium. International Chromium Development Association (ICDA), pp. 324.
Fersht A (1985) Enzyme Structure and Mechanism. New York: W. H. Freeman and Company.
Kaim W, Schwederski B (1994) Bioinorganic Chemistry: Inorganic Elements in the Chemistry of Life. Chichester: John Wiley & Sons.
Komori K, Wang P, Toda K, Ohyake H (1989) Factors affecting chromate reduction in Enterobacter cloacae strain HO1. Appl. Microbiol Biotechnol. 31: 567-570.
Lippard SJ, Berg JM (1994) Principles of Bioinorganic Chemistry. Mill Valley, CA: University Science Books.
Llovera S, Bonet R, Simon-Pujol MD, Congregado F (1993) Chromate reduction by resting cells of Agrobacterium radiobacter EPS-916. Appl. Environ. Microbiol. 59: 3516-3518.
Lloyd JR, Mabbett AN, Williams DR, Macaskie LE (2000) Metal reduction by sulphate-reducing bacteria: physiological diversity and metal specificity. Hydrometallurgy 59: 327-337.
Lloyd JR, Nolting HF, Sole VA, Bosecker K, Macaskie LE (1998) Technetium reduction and precipitation by sulfate-reducing bacteria. Geomicrobiol. J. 15: 45-58.
Lovley DR, Phillips EJP (1991) Reduction of uranium by Desulfovibrio desulfuricans. Appl. Environ. Microbiol. 58: 850-856.
Lovley DR, Phillips EJP (1994) Reduction of chromate by Desulfovibrio vulgaris and its c 3 cytochrome. Appl. Environ. Microbiol. 60: 726-728.
Ohtake H, Fujii E, Toda K (1990) Reduction of toxic chromate in an industrial effluent by the use of a chromate-reducing strain of Enterobacter cloacae. Environ. Technol. 11: 663-668.
Pattanapipitpaisal P, Brown NL, Macaskie LE (2001) Chromate reduction by Microbacterium liquefaciens immobilised in polyvinyl alcohol. Biotechnol. Lett. 23: 61-65.
Postgate J (1979) The Sulphate Reducing Bacteria. Cambridge, UK: Cambridge University Press.
Rollinson CL (1973) Chromium: the element. In: Bailar JC, Emeleus HJ, Nyholm SR, Trotman-Dickenson AF, eds. Comprehensive Inorganic Chemistry. Oxford: Pergamon Press Ltd, pp. 624-700.
Smith WL, Gadd GM (2000) Reduction and precipitation of chromate by mixed culture sulphate-reducing bacterial biofilms. J. Appl. Microbiol. 88: 983-991.
Suzuki T, Miyata N, Horitsu H, Kawai K, Takamizawa K, Tai Y, Okazakai M (1992) NAD(P)H-dependent chromium(VI) reductase of Pseudomonas ambigua G-1: a Cr(IV) intermediate is formed during the reduction of Cr(VI) to Cr(III). J. Bacteriol. 172: 5340-5345.
Wang Y, Shen H (1995) Bacterial reduction of hexavalent chromium. J. Indust. Microbiol. 14: 159-163.
Yong P, Macaskie LE (1999) The role of sulfate as competitive inhibitor of enzymatically-mediated heavy metal uptake by Citrobacter sp.: implications in the bioremediation of acid mine drainage water using biogenic phosphate precipitatant. J. Chem. Technol. Biotechnol. 74: 1149-1156.
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Mabbett, A.N., Macaskie, L.E. A novel isolate of Desulfovibrio sp. with enhanced ability to reduce Cr(VI). Biotechnology Letters 23, 683–687 (2001). https://doi.org/10.1023/A:1010352417399
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DOI: https://doi.org/10.1023/A:1010352417399