Abstract
A new and simple method was developed to recover paclitaxel from the extracellular culture medium of Taxus chinensis. More than 80% of paclitaxel in the medium was obtained by adding 7.8 mM MgSO4 or greater and then centrifuging. The concentration of baccatin III in the supernatant did not change after MgSO4 treatment while paclitaxel was precipitated in the pellet. This method was used to recover paclitaxel without baccatin III from the extracellular culture medium of Taxus species.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Choi HK, Kim SI, Son JS, Hong SS, Lee HS, Chung IS, Lee HJ (2000) Intermittent maltose feeding enhances paclitaxel production in suspension culture of Taxus chinensis cells. Biotechnol. Lett. 22: 1793-1796.
Fett-Netto AG, Zhang WY, DiCosmo F (1994) Kinetics of taxol production, growth, and nutrient uptake in cell suspensions of Taxus cuspidata. Biotechnol. Bioeng. 44: 205-210.
Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp. Cell Res. 50: 151-158.
Ketchum REB, Gibson DM, Croteau RB, Shuler ML (1999) The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol. Bioeng. 62: 97-105.
Kim JH, Hong SS (2000) Recovery of paclitaxel from suspension culture medium with hydrophobic resin. Korean J. Biotechnol. Bioeng. 15: 366-369.
Mirjalili N, Linden JC (1996) Methyl jasmonate induced production of taxol in suspension cultures of Taxus cuspidata: ethylene interaction and induction model. Biotechnol. Prog. 12: 110-118.
Pestchanker LJ, Roberts SC, Shuler ML (1996) Kinetics of taxol production and nutrient use in suspension cultures of Taxus cuspidata in shake flasks and a Wilson-type bioreactor. Enzyme Microb. Tech. 19: 256-260.
Seki M, Takeda M, Furusaki S (1995) Continuous production of taxol by cell culture of Taxus cuspidata. J. Chem. Eng. Japan 28: 488-490.
Suffness M, Wall ME (1995) Discovery and development of taxol. In: Suffness M, ed. Taxol: Science and Application. Boca Raton: CRC Press, pp. 3-25.
Wang HQ, Zhong JJ, Yu JT (1997) Enhanced production of paclitaxel in suspension cultures of Taxus chinensis by controlling inoculum size. Biotechnol. Lett. 19: 353-355.
Wickremesinhe ERM, Arteca RN (1994) Taxus cell suspension cultures: optimizing growth and production of taxol. J. Plant Physiol. 144: 183-188.
Yukimune Y, Tabata H, Higashi Y, Hara Y (1996) Methyl jasmonate-induced overproduction of paclitaxel and baccatin III in Taxus cell suspension cultures. Nature Biotechnol. 14: 1129-1132.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Son, JS., Choi, HK. & Hong, SS. Recovery of extracellular paclitaxel from suspension culture of Taxus chinensis by adding inorganic salts. Biotechnology Letters 23, 723–725 (2001). https://doi.org/10.1023/A:1010350902765
Issue Date:
DOI: https://doi.org/10.1023/A:1010350902765