Abstract
Production of nisin and pediocin were followed, respectively, in Lactococcus lactis subsp. lactis CECT 539 and Pediococcus acidilactici NRRL B-5627 grown with lactose and four different nitrogen sources. Neither NH4Cl nor glycine improved production of the bacteriocins. Both yeast extract and Casitone increased pediocin production from 55 BU ml−1 to 195 BU ml−1 and 185 BU ml−1, respectively. Nisin increased from 21 BU ml−1 to 74 BU ml−1 and 59 BU ml−1 with these nitrogen sources.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Akhnazarova S, Kafarov V (1982) Experiment Optimization in Chemistry and Chemical Engineering. Moscow: MIR Publ.
Baranova IP, Egorov NS (1967) Influence of different nitrogen compounds on growth of Streptococcus lactis and nisin formation. Microbiologia 36: 958-963.
Cabo ML, Murado MA, González MP, Pastoriza L (1999) A method for bacteriocin quantification. J. Appl. Microbiol. 87: 907-914.
De Jonge BLM, Chang Y-S, Xu N, Gage D (1996) Effect of exogenous glycine on peptidoglycan composition and resistance in a methicillin-resistant Staphylococcus aureus strain. Antimicrob. Agents Chemother. 40: 1498-1503.
De Vuyst L, De Poorter G, Vandamme EJ (1989) Nutritional and metabolic regulation of the nisin fermentation process. Med. Fac. Landbouww. Univ. Gent 54: 1501-1506.
De Vuyst L (1995) Nutritional factors affecting nisin production by Lactococcus lactis subsp. lactis NIZO 22186 in a synthetic medium. J. Appl. Bacteriol. 78: 28-33.
Goulhen F, Meghrous J, Lacroix C (1999) Production of a nisin Z/pediocin mixture by pH-controlled mixed-strain batch cultures in supplemented whey permeate. J. Appl. Microbiol. 86: 399-406.
Ray B (1992) Bacteriocins of starter culture bacteria as food biopreservative. In: Ray B, Daeschel M, eds. Food Biopreservatives of Microbial Origin, Vol. 8. Florida: CRC Press, pp. 177-205.
Yan TR, Lee CS (1997) Characterization of a partially purified bacteriocin, Fermentcin B, from Lactobacillus fermentum. Biotech. Lett. 19: 741-744.
Yang R, Ray B (1994) Factors influencing production of bacteriocins by lactic acid bacteria. Food Microbiol. 11: 281-291.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Guerra, N.P., Pastrana, L. Enhanced nisin and pediocin production on whey supplemented with different nitrogen sources. Biotechnology Letters 23, 609–612 (2001). https://doi.org/10.1023/A:1010324910806
Issue Date:
DOI: https://doi.org/10.1023/A:1010324910806