Abstract
A Lactococcus strain with strong antimicrobial activity was isolated from raw milk Manchego cheese during a survey on the production of bacteriocins by lactic acid bacteria present in raw milk cheeses. It was identified as Lactococcus lactis subsp. lactis, phenotypically by its morphological and physiological characteristics and genotypically by a PCR technique. When tested for tolerance to known bacteriocins produced by lactococci, it was shown to be resistant to nisin A and nisin Z. The presence of genes encoding nisin and lacticin 481 was revealed by PCR techniques with specific probes. Sequences of the respective PCR amplified fragments matched sequences reported for nisin Z and lacticin 481.
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Beimfohr C, Ludwig W, Schleifer KH (1997) Rapid genotypic differentiation of Lactococcus lactis subspecies and biovar. System. Appl. Microbiol. 20: 216-221.
Bogovic-Matijasic B, Rogelj I, Nes IF, Holo H (1998) Isolation and characterization of two bacteriocins of Lactobacillus acidophilus LF221. Appl. Microbiol. Biotechnol. 49: 606-612.
Cogan TM, Barbosa M, Beuvier E, Bianchi-Salvadori B, Cocconcelli PS, Fernández I, Gómez MJ, Gómez R, Kalantzopoulos G, Ledda A, Medina M, Rea MR, Rodríguez E (1997) Characterization of the lactic acid bacteria in artisanal dairy products. J. Dairy Res. 64: 409-421.
Contreras BGL, De Vuyst L, Devreese B, Busanyova K, Raymaeckers J, Bosman F, Sabol E, Vandamme EJ (1997) Isolation, purification, and amino acid sequence of lactobin A., one of the two bacteriocins produced by Lactobacillus amylovorus LMG P-13139. Appl. Environ. Microbiol. 63: 13-20.
De Vos WM, Mulders JWM, Siezen RJ, Hugenholtz J, Kuipers OP (1993) Properties of nisin Z and distribution of its gene, nisZ, in Lactococcus lactis. Appl. Environ. Microbiol. 59: 213-218.
De Vuyst L (1994) Nisin production variability between natural Lactococcus lactis subsp. lactis strains. Biotechnol. Lett. 16: 287-292.
Dodd HM, Horn N, Gasson MJ (1990) Analysis of the genetic determinant for production of the peptide antibiotic nisin. J. Gen. Microbiol. 136: 555-556.
Hanlin MB, Kalchayanand N, Ray P, Ray B (1993) Bacteriocins of lactic acid bacteria in combination have greater antibacterial activity. J. Food Prot. 56: 252-255.
Jiménez-Díaz R, Rios-Sànchez RM, Desmazeaud M, Ruiz-Barba JL, Piard J-C (1993) Plantaricins S and T, two new bacteriocins produced by Lactobacillus plantarum LPCO10 isolated from a green olive fermentation. Appl. Environ. Microbiol. 59: 1416-1424.
Krämer J, Brandis H (1975) Purification and characterization of two bacteriocins from Streptococcus faecium. J. Gen. Microbiol. 88: 93-100.
Martínez MI, Rodríguez E, Medina M, Hernández PE, Rodríguez JM (1998) Detection of specific bacteriocin-producing lactic acid bacteria by colony hybridization. J. Appl. Microbiol. 84: 1099-1103.
Moll GN, van der Akker E, Hauge HH, Nissen-Meyer J, Nes IF, Konings WN, Driessen AJM (1999) Complementary and overlapping selectivity of the two-peptide bacteriocins plantaricin EF and JK. J. Bacteriol. 181: 4848-4852.
Morgan S, Ross RP, Hill C (1995) Bacteriolytic activity caused by the presence of a novel lactococcal plasmid encoding lactococcins A, B, and M. Appl. Environ. Microbiol. 61: 2995-3001.
Mulders JWM, Boerrigter IJ, Rollema HS, Siezen RJ, De Vos WM (1991) Identification and characterization of the lantibiotic nisin Z, a natural nisin variant. Eur. J. Biochem. 201: 581-584.
Mulet-Powell N, Lacoste-Armynot AM, Viñas M, Simeon de Buochberg M (1998) Interactions between pairs of bacteriocins from lactic acid bacteria. J. Food Prot. 61: 1210-1212.
Piard J-C, Delorme F, Giraffa G, Commissaire J, Desmazeaud M (1990) Evidence for a bacteriocin produced by Lactococcus lactis CNRZ 481. Neth. Milk Dairy J. 44: 143-158.
Piard J-C, Muriana PM, Desmazeaud MJ, Klaenhammer TR (1992) Purification and partial characterization of lacticin 481, a lanthionine-containing bacteriocin, produced by Lactococcus lactis subsp. lactis CNRZ 481. Appl. Environ. Microbiol. 58: 279-284.
Piard J-C, Kuipers OP, Rollema HS, Desmazeaud MJ, De Vos WM (1993) Structure, organization, and expression of the lct gene for lacticin 481, a novel lantibiotic produced by Lactococcus lactis. J. Biol. Chem. 268: 16361-16368.
Remiger A, Eijsink VGH, Ehrmann MA, Sletten K, Nes IF, Vogel RF (1999) Purification and partial amino acid sequence of plantaricin 1.25α and 1.25β, two bacteriocins produced by Lactobacillus plantarum TMW1.25. J. Appl. Microbiol. 86: 1053-1058.
Revol-Junelles A-M, Mathis R, Krier F, Fleury Y, Delfour A, Lefebvre G (1996) Leuconostoc mesenteroides subsp. mesenteroides FR52 synthesizes two distinct bacteriocins. Lett. Appl. Microbiol. 23: 120-124.
Rincé A, Dufour A, Uguen P, Le Pennec J-P, Haras D (1997) Characterization of the lacticin 481 operon: the Lactococcus lactis genes lctF, lctE, and lctG encode a putative ABC transporter involved in bacteriocin immunity. Appl. Environ. Microbiol. 63: 4252-4260.
Rodríguez JM, Cintas LM, Casaus P, Horn N, Dodd HM, Hernandez PE, Gasson MJ (1995) Isolation of nisin-producing Lactococcus lactis strains from dry fermented sausages. J. Appl. Bacteriol. 78: 109-115.
Rodríguez E, González B, Gaya P, Nuñez M, Medina M (2000) Diversity of bacteriocins produced by lactic acid bacteria isolated from raw milk. Int. Dairy J. 10: 7-15.
Schillinger U, Lücke F-K (1989) Antibacterial activity of Lactobacillus sake isolated from meat. Appl. Environ. Microbiol. 55: 1901-1906.
Shiba T, Wakamiya T, Fukase K, Ueki Y, Teshima T, Nishikawa M (1991) Structure of the lanthionine peptides nisin, ancovenin and lanthiopeptin. In: Jung G, Sahl H-G, eds. Nisin and Novel Lantibiotics. Leiden: ESCOM Science Publishers BV, pp. 113-122.
van Belkum MJ, Hayema BJ, Geis A, Kok J, Venema G (1989) Cloning of two bacteriocin genes from a lactococcal bacteriocin plasmid. Appl. Environ. Microbiol. 55: 1187-1191.
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Garde, S., Rodríguez, E., Gaya, P. et al. PCR detection of the structural genes of nisin Z and lacticin 481 in Lactococcus lactis subsp. lactis INIA 415, a strain isolated from raw milk Manchego cheese . Biotechnology Letters 23, 85–89 (2001). https://doi.org/10.1023/A:1010316514641
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DOI: https://doi.org/10.1023/A:1010316514641