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Effects of gaseous NO2 on cells of Nitrosomonas eutropha previously incapable of using ammonia as an energy source

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Abstract

Cells of Nitrosomonas eutropha grown under anoxic conditions with hydrogen as electron donor and nitrite as electron acceptor were initially unable to oxidize ammonia (ammonium) and hydroxylamine when transferred to oxic conditions. Recovery of ammonia and hydroxylamine oxidation activity was dependent on the presence of NO2. Under oxic conditions, without addition of NO2, ammonia consumption started after 8 – 9 days, and small amounts of NO and NO2 were detectable in the gas atmosphere. Removing these nitrogen oxides by intensive aeration, ammonia oxidation activity decreased and broke off after 15 days. Addition of gaseous NO2 (25 ppm) led to a fast recovery of ammonia oxidation (3 days). Simultaneously, the arrangement of intracytoplasmic membranes (ICM) changed from circular to flattened vesicles, the protein pattern revealed an increase in the concentration of a 27 and a 30 kDa polypeptide, and the cytochrome c content increased significantly.

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Authors and Affiliations

  1. Institute for General Botany, Department of Microbiology, University of Hamburg, Ohnhorststraße 18, 22609, Hamburg, Germany

    Ingo Schmidt, Dirk Zart & Eberhard Bock

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  1. Ingo Schmidt
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  2. Dirk Zart
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  3. Eberhard Bock
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Correspondence to Ingo Schmidt.

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Schmidt, I., Zart, D. & Bock, E. Effects of gaseous NO2 on cells of Nitrosomonas eutropha previously incapable of using ammonia as an energy source. Antonie Van Leeuwenhoek 79, 39–47 (2001). https://doi.org/10.1023/A:1010269331350

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