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Comparative Study of Effects of Artificial Electron Donors on the AT-Band of Photosystem II Thermoluminescence

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Abstract

Extraction of the Mn-cluster from photosystem II (PS II) inhibits the main bands of thermoluminescence and induces a new AT-band at –20°C. This band is attributed to the charge recombination between acceptor QA − and a redoxactive histidine residue on the donor side of PS II. The effect of Mn(II) and Fe(II) cations as well as the artificial donors diphenylcarbazide and hydroxylamine on the AT-band of thermoluminescence was studied to elucidate the role of the redoxactive His residue in binding to the Mn(II) and Fe(II). At the Mn/PS II reaction center (RC) ratio of 90 : 1 and Fe/PS II RC ratio of 120 : 1, treatment with Mn(II) and Fe(II) causes only 60% inhibition of the AT-band. Preliminary exposure of Mn-depleted PS II preparations to light in the presence of Mn(II) and Fe(II) causes binding of the cations to the high-affinity Mn-binding site, thereby inhibiting oxidation of the His residue involved in the AT -band formation. The efficiency of the AT-band quenching induced by diphenylcarbazide and hydroxylamine is almost an order of magnitude higher than the quenching efficiency of Mn(II) and Fe(II). Our results suggest that the redox-active His is not a ligand of the high-affinity site and does not participate in the electron transport from Mn(II) and Fe(II) to YZ . The concentration dependences of the AT-band inhibition by Mn(II) and Fe(II) coincide with each other, thereby implying specific interaction of Fe(II) with the donor side of PS II.

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Authors and Affiliations

  1. School of Biology, Lomonosov Moscow State University, Moscow, 119899, Russia

    M. Yu. Kultisheva, E. R. Lovyagina, B. K. Semin & I. I. Ivanov

  2. School of Physics, Lomonosov Moscow State University, Moscow, 119899, Russia

    A. M. Kuznetsov & M. K. Solntsev

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  1. M. Yu. Kultisheva
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  2. E. R. Lovyagina
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  3. A. M. Kuznetsov
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  4. M. K. Solntsev
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  5. B. K. Semin
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Kultisheva, M.Y., Lovyagina, E.R., Kuznetsov, A.M. et al. Comparative Study of Effects of Artificial Electron Donors on the AT-Band of Photosystem II Thermoluminescence. Biochemistry (Moscow) 66, 715–720 (2001). https://doi.org/10.1023/A:1010248309383

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