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Use of introgression lines and zonal mapping to identify RAPD markers linked to QTL

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Abstract

RAPD primers were identified as giving parent-specific bands when screened with a set of introgression lines containing introgressed regions of Lycopersicon pennellii that encompass 5 quantitative trait loci affiliated with the production and composition of acylsugars, compounds associated with insect resistance. Primers giving L. pennellii introgression specific bands were zonally mapped to identify bands affiliated with the quantitative trait target and flanking regions using subsets of 7 to 16 F2 individuals which contained small overlapping segments (zones) of the L. pennellii genome spanning those regions. Seventeen RAPD primers, agt-related primers, and an agt clone were then used in mapping the complete F2 population of 144 individuals. This work resulted in the identification of RAPD markers for three of the 5 quantitative trait loci and the construction of an integrated RAPD/RFLP genomic map for tomato (Lycopersicon esculentum x L. pennellii LA716) of 111 RAPD and 8 acylglucose transferase related markers added to a framework map of 150 RFLP markers.

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  1. Dept. of Plant Breeding and Biometry, Cornell University, Ithaca, NY, 14853, USA

    K.L. McNally & M.A. Mutschler

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  1. K.L. McNally
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  2. M.A. Mutschler
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McNally, K., Mutschler, M. Use of introgression lines and zonal mapping to identify RAPD markers linked to QTL. Molecular Breeding 3, 203–212 (1997). https://doi.org/10.1023/A:1009657122293

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