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Development of coupling-repulsion-phase SCAR markers diagnostic for the sugar beet Rr1 allele conferring resistance to rhizomania

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Abstract

In sugar beet genotypes with the ‘Holly’ type of resistance to rhizomania, a disease due to infection of the beet necrotic yellow vein virus (BNYVV), the major gene rrl is responsible for resistance. Twelve RAPD markers linked to rrl were selected by BSA and mapped on linkage group IV using a segregating population previously analysed by the same group. Markers F61050 and N9600 were tightly linked, respectively in coupling and repulsion, to the Rrl allele (recombination values of 1.4 cM for both markers). After sequencing the products amplified by F61050 and N9600, new PCR primers were used to generate the two SCAR markers F6 and N9. The simultaneous use of these markers in a PCR reaction allows the correct fingerprinting of rrl rrl, Rrl rrl and Rrl Rrl sugar beet plants in populations segregating for the ‘Holly’ resistance. In a group of sugar beet elite lines containing the ‘Holly’ type of rhizomania resistance, SCAR F6 is always present whereas the SCAR N9 fragment is absent. Thus, in marker-assisted selection with coupling-repulsion-phase markers, SCAR F6 can be used in combination with N9, or together with any other RAPD marker linked in repulsion to the Rrl allele.

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Authors and Affiliations

  1. Max-Planck-Institut für Züchtungsforschung, Carl-von-Linné-Weg 10, 50829, Köln, Germany

    Ellen Barzen, Rainer Stahl, Elke Fuchs & Francesco Salamini

  2. KWS Kleinwanzlebener Saatzucht AG, Grimsehlstrasse 31, 37555, Einbeck, Germany

    Dietrich C. Borchardt

Authors
  1. Ellen Barzen
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  2. Rainer Stahl
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  3. Elke Fuchs
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  4. Dietrich C. Borchardt
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  5. Francesco Salamini
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Barzen, E., Stahl, R., Fuchs, E. et al. Development of coupling-repulsion-phase SCAR markers diagnostic for the sugar beet Rr1 allele conferring resistance to rhizomania. Molecular Breeding 3, 231–238 (1997). https://doi.org/10.1023/A:1009626214058

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  • DOI: https://doi.org/10.1023/A:1009626214058

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