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A rapid PCR-aided selection of a rice line containing the Rf-1 gene which is involved in restoration of the cytoplasmic male sterility

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Abstract

Cytoplasmic male sterility (CMS) is widely known in higher plants, the mechanism of which is believed to involve incompatibility between nuclei and cytoplasms. In rice lines with the CMS trait, fertility is restored by the aid of a nuclear-encoded gene, Rf-1, whose locus has been determined in chromosome 10. We found a particular PCR-amplified fragment, designated fL601, that specifically amplified using the DNAs from Rf-1 lines tested as templates. RFLP mapping of the fL601 locus revealed that there are two loci for the fL601, and that both are tightly linked to the Rf-1 locus. Progeny analysis also showed high frequency of their co-segregation. Southern analysis of the genomic DNA demonstrated that the Rf-1 lines shared a unique sequence in the fL601 region. These results enabled us to construct a system for specific detection of the corresponding regions. Utilizing this detection system, we established a simple PCR-mediated selection method for the Rf-1 lines, which may facilitate the breeding for hybrid rice.

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Authors and Affiliations

  1. Life Science Institute, Mitsui Toatsu Chemicals, Inc., Togo 1144, Mobara, 297, Japan

    Norio Ichikawa, Akiko Inagaki, Atsushi Nakamura, Yoko Koshino, Yumi Yokozeki, Masaaki Oka, Shiro Samoto, Hiromori Akagi, Tatsuhito Fujimura & Hiroaki Shimada

  2. National Institute of Agrobiological Resources, Tsukuba, 305, Japan

    Noaki Kishimoto & Kenichi Higo

  3. College of Agriculture, University of Ryukyus, Okinawa, 903-01, Japan

    Choyu Shinjyo

  4. Mitsui Plant Biotechnology Research Institute, Sengen 2-1-6, Tsukuba, 305, Japan

    Norio Ichikawa

  5. CSIRO Division of Plant Industry, GPO Box 1600, Canerra, ACT 2601, Australia

    Noaki Kishimoto

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  1. Norio Ichikawa
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  2. Noaki Kishimoto
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  3. Akiko Inagaki
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  4. Atsushi Nakamura
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  5. Yoko Koshino
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  6. Yumi Yokozeki
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  7. Masaaki Oka
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  8. Shiro Samoto
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  9. Hiromori Akagi
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  10. Kenichi Higo
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  11. Choyu Shinjyo
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  12. Tatsuhito Fujimura
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  13. Hiroaki Shimada
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Corresponding author

Correspondence to Hiroaki Shimada.

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Ichikawa, N., Kishimoto, N., Inagaki, A. et al. A rapid PCR-aided selection of a rice line containing the Rf-1 gene which is involved in restoration of the cytoplasmic male sterility. Molecular Breeding 3, 195–202 (1997). https://doi.org/10.1023/A:1009601007175

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