Abstract
Effects of internal rotations of chromophores under the energy transfer interaction in proteins on the time-resolved fluorescence were examined by numerical calculations. Expressions used for the calculations are based on the approximations that the energy transfer takes place according to Foöurster's mechanism and the rotational motions of the energy donor and acceptor along the surfaces of cones are described by a set of rotational diffusion equations. The intensity decay of the donor depended a little on the rotational diffusion coefficient of the donor in some cases, while that of the acceptor did very little. Anisotropy of the donor decayed faster as the diffusion coefficient of the donor increased. Anisotropy decay of the acceptor markedly depended not only on the mutual configuration of the pair in the protein, but also on the diffusion coefficient of the donor. The dependence of the time-resolved fluorescence on the diffusion coefficient of the acceptor was not as great as that of the donor.
Similar content being viewed by others
REFERENCES
I. Z. Steiberg (1971) Annu. Rev. Biochem. 40, 83-114.
L. Stryer (1978) Annu. Rev. Biochem. 47, 819-846.
T. Forster (1948) Ann. Phys. 2, 55-75.
R. E. Dale and J. Eisinger (1975) in R. F. Chen and H. Edelhoch (Eds.), Biochemical Fluorescence: Concept, Vol. 1, Marcel Dekker, New York, pp. 115-284.
J. M. Beechem and L. Brand (1985) Annu. Rev. Biochem. 54, 43-71.
R. D. Spencer and G. Weber (1970) J. Chem. Phys. 52, 1654-1663.
F. Tanaka and N. Mataga (1979) Photochem. Photobiol. 29, 1091-1097.
A. Szabo (1984) J. Chem. Phys. 81, 150-167.
M. N. Berberan-Santos and B. Valeur (1991) J. Chem. Phys. 95, 8048-8055.
J. J. Fisz (1996) Chem. Phys. Lett. 262, 495-506.
F. Tanaka and N. Mataga (1982) Biophys. J. 39, 129-140.
P.-O. Westlund and H. Wennerstrom, J. Chem. Phys. 99, 6583-6589.
I. Fedchenia and P.-O. Westlund (1994) Phys. Rev. E 50, 555-565.
L. B.-A. Johanson, P. Edman, and P.-O. Westlund (1996) J. Chem. Phys. 105, 10896-10904.
F. Tanaka (1998) J. Chem. Phys. 109, 1084-1092.
Y. Y. Gottlieb and Ph. Wahl (1963) J. Chim. Phys. 60, 849-856.
Ph. Wahl and G. Weber (1967) J. Mol. Biol. 30, 371-382.
F. Tanaka and N. Mataga (1992) in N. Mataga, T. Okada, and H. Masuhara (Eds.), Dynamics and Mechanisms of Photoinduced Electron Transfer and Related Phenomena, Elsevier, Tokyo, pp. 501-512.
F. Tanaka, N. Tamai, N. Mataga, B. Tonomura, and K. Hiromi (1994) Biophys. J. 67, 874-880.
F. Tanaka and N. Mataga (1987) Biophys. J. 51, 487-495.
M. Karplus and J. A. McCammon (1983) Annu. Rev. Biochem. 52, 263-300.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Tanaka, F. Effects of Internal Rotations on the Time-Resolved Fluorescence in a Bichromophoric Protein System Under the Energy Transfer Interaction. Journal of Fluorescence 10, 13–20 (2000). https://doi.org/10.1023/A:1009427327771
Issue Date:
DOI: https://doi.org/10.1023/A:1009427327771