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Clinical Assisted Reproduction: Prolonged Culture of Human Cryopreserved Embryos with Recombinant Human Leukemia Inhibitory Factor

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Abstract

Purpose: To evaluate the efficiency of recombinant humanleukemia inhibitory factor (LIF) in the prolonged culture ofhuman cryopreserved-thawing embryos.

Methods: After thawing, all embryos were divided into fourgroups: (1) Human tubal fluid (HTF), (2) HTF + LIF, (3)M3TH medium, and (4) M3TH medium plus LIF. Followingprolonged culture, embryo development in each groupwas compared.

Results: In embryo development from about the 2– to 4–cellto 9– to 16–cell stage, there were nonsignificant differencesbetween each group. There was lower morula formation ratein group 1 (6.9%) than those in other groups (23.2%, 19.7%,23.1%). The lower blastocyst formation in group 1 and 3(0%, 0%) than those in group 2 and 4 (11.0%, 12.8%)were noted.

Conclusions: LIF is beneficial for preimplantation embryos.LIF does not influence the early embryo development.LIF-supplemented HTF provided a similar culture environmentfor thawing embryos as LIF-supplemented M3TH medium.

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REFERENCES

  1. Tsai HD, Chang CC, Hsieh YY, Lo HY, Hsu LW, Chang SC: Recombinant human leukemia inhibitory factor enhances the development of preimplantation mouse embryo in vitro. Fertil Steril 1999;71:722–725

    Google Scholar 

  2. Virginia R: Coculture cells that express leukemia inhibitory factor (LIF) enhance mouse blastocyst development in virto. J Assist Reprod Gen 1995;12:153–156

    Google Scholar 

  3. Marquant-Le Guinne B, Humbolt P, Guillon N, Thibier M: Murine LIF improves the development of IVF cultured bovine morulae (Abstract). J Reprod Fertil 1993;12:61

    Google Scholar 

  4. Fry RC, Batt PA, Fairclough RJ, Parr RA: Human leukemia inhibitory factor improves the viability of ovine embryos. Biol Reprod 1992;46:470–474

    Google Scholar 

  5. Dunglison GF, Barlow DH, Sargent IL: Leukaemia inhibitory factor significantly enhances the blastocyst formation rates of human embryos cultured in serum-free medium. Hum Reprod 1996;11:191–196

    Google Scholar 

  6. Jurisicova A, Ben-Chetrit A, Varmuza SL, Casper RF: Recombinant human leukemia inhibitory factor does not enhance in vitro human blastocyst formation. Fertil Steril 1995;64:999–1002

    Google Scholar 

  7. Tasi HD, Chen CM, Lo HY, Chang CC: Subcutaneous low does leuprolide acetate depot versus leuprolide acetate for women undergoing ovarian stimulation for in vitro fertilization. Hum Reprod 1995;10:2909–2912

    Google Scholar 

  8. Hsieh YY, Tsai HD, Chang CC, Chang CC, Lo HY, Lai CH: Ultra-rapid cryopreservation of human embryos-1582 embryo experience. Fertil Steril 1999;72:253–256

    Google Scholar 

  9. Lai AC, Lin BP, Chang CC, Tsai HD, Hwang VW, Lo HY: Pregnancies after transfer of ultrarapidly frozen human embryos. J Assist Reprod Genet. 1996;13:625–628

    Google Scholar 

  10. Chang SC, Wang F, Hsu LW: Expression of recombinant human leukemia inhibitory factor (rhLIF) in Escherichia coli. Med J CMCH 1997;2:6–11

    Google Scholar 

  11. Bhatt H, Brunet LJ, Stewart CL: Uterine expression of leukemia inhibitory factor coincides with the onset of blastocyst implantation. Proc Natl Acad Sci USA 1991;88:11408–11412

    Google Scholar 

  12. Stewart CL: Leukemia inhibitory factor and the regulation of preimplantation development of the mammalian embryo. Mol Reprod Dev 1994;39:233–238

    Google Scholar 

  13. Michell MH, Swanson RJ, Hodgen GD, Oehninger S: Enhancement of in vitro murine embryo development by recombinant leukemia inhibitory factor. J Soc Gynecol Invest 1994;1:215–219

    Google Scholar 

  14. Menezo YJR, Guerin JF, Czyba JC: Improvement of human early embryo development in vitro by coculture on monolayers of Vero cells. Biol Reprod 1990;42:301–306

    Google Scholar 

  15. Gardner DK, Lane M: Alleviation of the 2–cell block and development to the blastocyst of CF1 mouse embryos: role of amino acids, EDTA, and physical factors. Hum Reprod 1996;11:2703–2712

    Google Scholar 

  16. Lane M, Gardner DK: Differential regulation of mouse embryo development and viability by amino acids. J Reprod Fertil 1997;109:153–164

    Google Scholar 

  17. Bolton VN, Hawes SM, Taylor CT: Development of spare human preimplantation embryos in vitro: An analysis of the correlations among gross morphology, cleavage rates, and development to the blastocyst. J in Vitro Fertil Embryo Transf 1989;6:30–35

    Google Scholar 

  18. Quinn P, Kerlin JF, Warnes GM: Improved pregnancy rate in human in vitro fertilization with the use of a medium based on the composition of human tubal fluid. Fertil Steril 1985;44:493–498

    Google Scholar 

  19. Jones GM, Trounson AO, Gardner DK, Kausche A, Lolatgis N, Wood C: Evolution of a culture protocol for successful blastocyst development and pregnancy. Hum Reprod 1998;13:169–177

    Google Scholar 

  20. Dale PO, Kjekshus E, Tanbo T, Holst N, Abyholm T: Clinical outcome of day 3 versus day 2 embryo transfer using serum-free culture media. Abstract of the 10th Annual Meeting of the ESHRE, Brussels 1994 (No. 068)

  21. Forsdahl F, Bertheussen K, Bungum LJ, Bungum MH, Willumsen J, Maltau JM: Astudy of extended culture time with embryo replacement of the morula and blastocyst stage. Abstract of the 10th Annual Meeting of ESHRE, Brussels 1994 (No. 375)

  22. Gardner DK, Vella P, Lane M, Wagley L, Schlenker T, Schoolcraft WB: Culture and transfer of human blastocysts increases implantation rates and reduces the need for multuple embryo transfers. Fertil Steril 1998;69:84–88

    Google Scholar 

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology, China Medical College Hospital, Taichung, Taiwan

    Yao-Yuan Hsieh, Horng-Der Tsai, Chi-Chen Chang, Li-Wei Hsu, Su-Chen Chang & Hui-Yu Lo

  2. Department of Obstetrics and Gynecology, China Medical College Hospital, No. 2 Yuh-Der Road, Taichung, Taiwan

    Horng-Der Tsai

  3. Department of Medical Research, China Medical College, Taichung, Taiwan

    Su-Chen Chang

Authors
  1. Yao-Yuan Hsieh
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  2. Horng-Der Tsai
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  3. Chi-Chen Chang
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  4. Li-Wei Hsu
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  5. Su-Chen Chang
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  6. Hui-Yu Lo
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Supported by China Medical College Hospital, Taiwan

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Hsieh, YY., Tsai, HD., Chang, CC. et al. Clinical Assisted Reproduction: Prolonged Culture of Human Cryopreserved Embryos with Recombinant Human Leukemia Inhibitory Factor. J Assist Reprod Genet 17, 131–134 (2000). https://doi.org/10.1023/A:1009426303742

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  • DOI: https://doi.org/10.1023/A:1009426303742

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