Skip to main content
SpringerLink
Log in

Silver staining as a new banding technique to identify aphid chromosomes

  • Published:
Chromosome Research Aims and scope Submit manuscript

Abstract

The karyotype of the aphid Aphis sambuci has been studied by means of C-banding, fluorochrome staining and AgNO3 staining. Heterochromatic areas are confined exclusively to the X chromosomes. Interestingly, silver staining produces a clear-cut longitudinal differentiation of all chromosomes, which allows reliable identification of homologues.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Goodpasture C, Bloom SE (1975) Visualization of nucleolar-organizer regions in mammalian chromosomes using silver staining. Chromosoma 53: 37–50.

    Article  PubMed  Google Scholar 

  • Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36: 1014–1015.

    PubMed  Google Scholar 

  • Manicardi GC, Gautam DC (1994) Cytogenetic investigations on the holocentric chromosomes of Tetraneurella akinire (Sasaki) (Homoptera Pemphigidae). Caryologia 47: 159–165.

    Google Scholar 

  • Manicardi GC, Gautam DC, Bizzaro D, Guicciardi E, Bonvicini Pagliai AM, Bianchi U (1991a) Chromosome banding in Aphids: G, C, AluI, and HaeIII banding patterns in Megoura viciae (Homoptera, Aphididiae). Genome 34: 661–665.

    Google Scholar 

  • Manicardi GC, Bizzaro D, Bonvicini Pagliai AM, Bianchi U (1991b) Characterization of Acyrthosiphon pisum Harris (Homoptera: Aphididae) karyotype using G and C banding. Proceedings of the XVI Italian Congress of Entomology, Bari/Martina Franca, Italy, 23-28 September 1991. Bari: Industria Grafica Laterza, pp 513–517.

    Google Scholar 

  • Manicardi GC, Bizzaro D, Galli E, Bianchi U (1996) Heterochromatin heterogeneity in the holocentric X chromatin of Megoura viciae (Homoptera, Aphididae). Genome 39: 465–470.

    Google Scholar 

  • Schweizer D, Loidl J (1987) A model for heterochromatin disperse and the evolution of C-band patterns. In: Stahl A, Luciani J, Vagner-Capodano AM, eds. Chromosomes Today, Vol. 9. London: Allen & Unwin, pp 61–74.

    Google Scholar 

  • Sumner AT (1972) A simple technique for demonstrating centromeric heterochromatin. Exp Cell Res 75: 304–306.

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Dipartimento di Biologia Animale, Universita di Modena, Viale Berengario 14, 41100, Modena, Italy

    Gian Carlo Manicardi, Davide Bizzaro, Mauro Mandrioli & Umberto Bianchi

Authors
  1. Gian Carlo Manicardi
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Davide Bizzaro
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Mauro Mandrioli
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Umberto Bianchi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Manicardi, G.C., Bizzaro, D., Mandrioli, M. et al. Silver staining as a new banding technique to identify aphid chromosomes. Chromosome Res 6, 55–57 (1998). https://doi.org/10.1023/A:1009270525511

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1009270525511

Search

Navigation