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Unusually high amount of inactive ribosomal DNA in the grasshopper Stauroderus scalaris

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Abstract

Fluorescence in-situ hybridization (FISH) was employed to determine the chromosomal location of the ribosomal DNA cistrons in spermatocytes of two populations of the grasshopper Stauroderus scalaris. The results showed that paracentromeric C-bands, which in this species constitute about 50% of the total chromatin, contain substantial amounts of rDNA in all chromosomes. However, silver impregnation showed the presence of a single active nucleolus organizing region (NOR) in chromosome 3 of primary spermatocytes, indicating an extremely high amount of silent rDNA across the whole genome of this species in the two geographically distant populations analysed. The significance of such an unusual phenomenon is discussed.

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References

  • Alonso-Blanco C, Pendás R, García-Suarez R, Roca A, Goicoechea PG, Giráldez R (1994) Physical mapping of 5S rDNA reveals a new locus on 3R and unexpected complexity in a rye translocation used in chromosome mapping. Chromosoma 103: 331-337.

    PubMed  CAS  Google Scholar 

  • Cabrero J, Camacho JPM (1985) A spontaneous interchange heterozygote mosaic in the grasshopper Stauroderus scalaris: interchromosomal chiasma effects. Heredity 54: 235-243.

    Google Scholar 

  • Cabrero J, Camacho JPM (1986) Cytogenetic studies in gomphocerine grasshoppers. II. Chromosomal location of active nucleolar organizer regions. Can J Genet Cytol 28: 540-544.

    Google Scholar 

  • Cabrero J, Navas-Castillo J, Camacho JPM (1986) Effects of supernumerary chromosome segments on the activity of nucleolar organiser regions in the grasshopper Chorthippus binotatus. Chromosoma 93: 375-380.

    Article  Google Scholar 

  • Cabrero J, Alché JD, Camacho JPM (1987) Effects of B chromosomes on the activity of nucleolar organizer regions in the grasshopper Eyprepocnemis plorans: activation of a latent nucleolar organizer region on a B chromosome fused to an autosome. Genome 29: 116-121.

    Google Scholar 

  • Camacho JPM, Cabrero J, Viseras E, López-León MD, Navas-Castillo J, Alché JD (1991) G-banding in two species of grasshoppers and its relationship to C, N and fluorescence banding techniques. Genome 34: 638-643.

    Google Scholar 

  • Castilho A, Queiroz A, Neves N, Barao A, Silva M, Viegas W (1995) The development stage of inactivation of rye origin ribosomal-RNA genes in the embryo and endosperm of wheat-rye F1 hybrids. Chrom Res 3: 169-174.

    Article  PubMed  CAS  Google Scholar 

  • Cermeño MC, Orellana J, Santos JL, Lacadena JR (1984) Nucleolar organizer activity in wheat, rye and derivatives analyzed by a silver staining procedure. Chromosoma 89: 370-376.

    Article  Google Scholar 

  • Cermeño MC, Friebe B, Zeller JF, Krolow KD (1987) Nucleolar competition in different (A/B) (A/B) RR and DDRR tetraploid triticales. Heredity 58: 1-4.

    Google Scholar 

  • Corey HI (1933) Chromosome studies in Stauroderus (an Orthopteran). J Morph 55: 313-347.

    Article  Google Scholar 

  • Cuadrado A, Jouve N, Heslop-Harrison JS (1995) Physical mapping of the 5S rRNA multigene family in 6x triticale and rye: identification of a new rye locus. Genome 38: 623-626.

    CAS  PubMed  Google Scholar 

  • Delgado M, Moraiscecilio L, Neves N, Jones RN, Viegas W (1995) The influence of B-chromosomes on rDNA organization in rye interphase nuclei. Chrom Res 3: 487-491.

    Article  PubMed  CAS  Google Scholar 

  • Gerlach WL, Bedbrook JR (1979) Cloning and characterization of ribosomal RNA genes from wheat and barley. Nucleic Acids Res 7: 1869-1885.

    PubMed  CAS  Google Scholar 

  • Goodpasture C, Bloom SE (1975) Visualization of nucleolar organizer regions in mammalian chromosomes using silver staining. Chromosoma 53: 37-50.

    Article  PubMed  CAS  Google Scholar 

  • Hizume M, Sato S, Tanaka A (1980) A highly reproducible method of nucleolus organizer regions staining in plants. Stain Technol 55: 87-90.

    PubMed  CAS  Google Scholar 

  • Hofgärtner FJ, Schmid M, Krone W, Zenzes MT, Engel W (1979) Pattern of activity of nucleolus organizers during spermatogenesis in mammals as analyzed by silver-staining, Chromosoma 71: 197-216.

    Article  PubMed  Google Scholar 

  • Hubbell HR (1985) Silver staining as an indicator of active ribosomal genes. Stain Technol 60: 285-294.

    PubMed  CAS  Google Scholar 

  • Jiménez R, Burgos M, Díaz de la Guardia R (1988) A study of the Ag-staining significance in mitotic NORs. Heredity 60: 125-127.

    PubMed  Google Scholar 

  • John B (1976) Myths and mechanisms of meiosis. Chromosoma 54: 295-325.

    Article  PubMed  CAS  Google Scholar 

  • Kusaba M, Tsuge T (1995) Phylogeny of Alternaria fungi to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA. Curr Genet 28: 491-498.

    Article  PubMed  CAS  Google Scholar 

  • López-León MD, Cabrero J, Camacho JPM (1991) A nucleolus organizer region in a B chromosome inactivated by DNA methylation. Chromosoma 100: 134-138.

    Article  Google Scholar 

  • López-León MD, Neves N, Schwarzacher T, Heslop-Harrison JS, Hewitt GM, Camacho JPM (1994) Possible origin of a B chromosome deduced from its DNA composition using double FISH technique. Chrom Res 2: 87-92.

    Article  PubMed  Google Scholar 

  • López-León MD, Cabrero J, Camacho JPM (1995) Changes in NOR activity pattern in the presence of supernumerary heterochromatin in the grasshopper Eyprepocnemis plorans. Genome 38: 68-74.

    PubMed  Google Scholar 

  • Marchi A, Pili E (1994) Ribosomal RNA genes in mosquitoes: localization by fluorescence in situ hybridization (FISH). Heredity 72: 599-605.

    PubMed  CAS  Google Scholar 

  • Meunier-Rotival M, Cortadas J, Macaya G, Bernardi G (1979) Isolation and organization of calf ribosomal DNA. Nucleic Acids Res 6: 2109-2123.

    PubMed  CAS  Google Scholar 

  • Meyne J, Hirai H, Imai HT (1995) FISH analysis of the telomere sequences of bulldog ants (Myrmecia: Formicidae). Chromosoma 104: 14-18.

    PubMed  CAS  Google Scholar 

  • Miller DA, Dev VG, Tantravahi R, Miller OJ (1976) Supression of human nucleolus organizer activity in mouse-human somatic hybrid cells. Exp Cell Res 101: 235-243.

    Article  PubMed  CAS  Google Scholar 

  • Rafferty JA, Fletcher HL (1992) Sequence analysis of a family of highly repeated DNA units in Stauroderus scalaris (Orthoptera). Int J Genom Res 1: 1-16.

    CAS  Google Scholar 

  • Ritland C, Straus A (1993) High evolutionary divergence of the 5.8S ribosomal DNA in Mimulus glaucescens. Plant Mol Biol 22: 691-696.

    Article  PubMed  CAS  Google Scholar 

  • Rufas JS, Gosálvez J, López-Fernández C, Cardoso H (1983) Complete dependence between Ag NORs and C-positive heterochromatin revealed by simultaneous Ag-NOR C-banding method. Cell Biol Int Rep 7: 275-281.

    Article  PubMed  CAS  Google Scholar 

  • Rufas JS, Iturra P, de Souza W, Esponda P (1982) Simple silver staining procedure for the localization of nucleolus and nucleolar organizer under light and electron microscopy. Arch Biol 93: 267-274.

    Google Scholar 

  • Sánchez A, Jiménez R, Burgos M, Stitou S, Zurita F, Díaz de la Guardia R (1995) Cytogenetic peculiarities in the Algerian hedgehog: silver stains not only NORs but also heterochromatic blocks. Heredity 75: 10-16.

    PubMed  Google Scholar 

  • Santos J, Sentís C, García-Rodríguez J, Férnandez-Piqueras J (1990) Latent NORs in the species Pycnogaster cucullata (Orthoptera). Heredity 65: 7-10.

    Google Scholar 

  • Silva M, Queiroz A, Neves N et al. (1995) Reprograming of rye rDNA triticale during microsporogenesis. Chrom Res 3: 492-96.

    Article  PubMed  CAS  Google Scholar 

  • Suja JA, Antonio C, Gónzalez-García JM, Rufas JS (1993) Supernumerary heterochromatic segments associated with the nucleolar chromosomes of Pyrgomorpha conica (Orthoptera) contain methylated rDNA sequences. Chromosoma 102: 491-499.

    Article  PubMed  CAS  Google Scholar 

  • Varley JM, Morgan GT (1978) Silver staining of the lampbrush chromosomes of Triturus cristatus carnifex. Chromosoma 67: 233-244.

    Google Scholar 

  • Zatsepina OV, Schofer C, Weipoltshammer K et al. (1996) The RNA-polymerase-I transcription factor UBF and rDNA are located at the same major sites in both interphase and mitotic pig embryonic kidney. Cytogenet Cell Genet 73: 274-278.

    Article  PubMed  CAS  Google Scholar 

  • Zurita F, Jiménez R, Burgos M, Díaz de la Guardia R (1998) Sequential silver staining and in situ hybridization reveal a direct association between rDNA levels and the expression of homologous nucleolar organizing regions: a hypothesis for NOR structure and function. J Cell Sci 111: 1433-1439.

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Departamento de Genetica, Universidad de Granada, E-18071, Granada, Spain

    M. D. Lopez-Leon, J. Cabrero & J. P. M. Camacho

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  1. M. D. Lopez-Leon
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  2. J. Cabrero
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  3. J. P. M. Camacho
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Lopez-Leon, M.D., Cabrero, J. & Camacho, J.P.M. Unusually high amount of inactive ribosomal DNA in the grasshopper Stauroderus scalaris. Chromosome Res 7, 83–88 (1999). https://doi.org/10.1023/A:1009270310092

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  • DOI: https://doi.org/10.1023/A:1009270310092

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