Abstract
The meiotic behaviour of the X chromosome and one autosomal pair of the heteropteran Triatoma infestans was analysed by means of C-banding plus DAPI staining. At first metaphase, the X univalent is oriented with its long axis parallel to the equatorial plate, which suggests a holocentric interaction with the spindle fibres. After this initial orientation, kinetic activity is restricted to one of both chromatid ends. The election of the active chromatid end is random and it is independent of the end selected in the sister chromatid. At second metaphase, the X and Y chromatids associate side by side forming a pseudobivalent. After that, the kinetic activity is again restricted to either of both chromosomal ends in a random fashion. At first metaphase, the fourth autosomal bivalent shows two alternative random orientations depending on the chromosome end showing kinetic activity (DAPI positive or opposite). At second metaphase, half bivalents are oriented with their long axis parallel to the equatorial plate. Three different segregation patterns are observed. The kinetic activity can be localised: (i) in the end with the DAPI signal (46.9%), (ii) in the opposite end (44.6%) or (iii) in one DAPI-positive end in one chromatid and in the opposite end in the other one (8.5%). The existence of the last pattern indicates that the same end can show kinetic activity during both meiotic divisions. Our results provide new information on the comparative meiotic behaviour of autosomes and sex chromosomes in holocentric systems.
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Albertson DG, Thomson JN (1993) Segregation of holocentric chromosomes at meiosis in the nematode Caenorhabditis elegans. Chromosome Res 1: 15–26.
Bella JL, Gosálvez J (1994) Banding human chromosomes using a combined C-banding-fluorocrome staining technique. Biotech Histochem 69: 243–248.
Buck RC (1967) Mitosis and meiosis in Rhodnius prolixus: the fine structure of the spindle and diffuse kinetochore. J Ultrastruct Res 18: 489–501.
Camacho JPM, Belda J, Cabrero J (1985) Meiotic behaviour of the holocentric chromosomes of Nezara viridula (Insecta, Heteroptera) analysed by C-banding and silver impregnation. Can J Genet Cytol 27: 491–497.
Comings DE, Okada TA (1972) Holocentric chromosomes in Oncopeltus: kinetochore plates are present in mitosis but absent in meiosis. Chromosoma 37: 177–192.
González-García JM, Benavente R, Rufas JS (1996a) Cytochemical and immunocytochemical characterization of kinetochores in the holocentric chromosomes of Graphosoma italicum. Eur J Cell Biol 70: 352–360.
González-García JM, Antonio C, Suja JA, Rufas JS (1996b). Meiosis in holocentric chromosomes: kinetic activity is randomly restricted to the chromatid ends of sex univalents in Graphosoma italicum (Heteroptera). Chromosome Res 4: 124–132.
Hughes-Schrader S, Schrader F (1961) The kinetochore of the Hemiptera. Chromosoma 12: 327–350.
Nokkala S (1985) Restriction of kinetic activity of holokinetic chromosomes in meiotic cells and its structural basis. Hereditas 102: 85–88.
Nokkala S, Nokkala C (1997) The absence of chiasma terminalization and inverted meiosis in males and females of Myrmus miriformis Fn. (Corizidae, Heteroptera). Heredity 78: 561–566.
Pérez R, Panzera F, Page J, Suja JA, Rufas JS (1997) Meiotic behaviour of holocentric chromosomes: orientation and segregation of autosomes in Triatoma infestans (Hemiptera: Reduviidae). Chromosome Res 5: 47–56.
Ruthmann A, Dahlberg R (1976) Pairing and segregation of the sex chromosomes in X1X2-males of Dysdercus intermedius with a note on the kinetic organization of heteropteran chromosomes. Chromosoma 54: 89–97.
Schrader F (1935) Notes on the mitotic behaviour of long chromosomes. Cytologia 6: 422–430.
Schreiber G, Pellegrino J (1950) Eteropicnosi di autosomi come possible meccanismo di speciazione (Ricerche citologiche su alcuni Emitteri neotropici). Sci Genet 3: 215–226.
Solari AJ (1979) Autosomal synaptonemal complexes and sex chromosomes without axes in Triatoma infestans (Reduviidae, Hemiptera). Chromosoma 72: 225–240.
White MJD (1973) Animal Cytology and Evolution, 3rd edn. Cambridge: Cambridge University Press, London.
Wolfe SL, John B (1965) The organization and ultrastructure of male meiotic chromosomes in Oncopeltus fasciatus. Chromosoma 17: 85–103.
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Pérez, R., Rufas, J.S., Suja, J.A. et al. Meiosis in Holocentric Chromosomes: Orientation and Segregation of an Autosome and Sex Chromosomes in Triatoma infestans (Heteroptera). Chromosome Res 8, 17–25 (2000). https://doi.org/10.1023/A:1009266801160
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DOI: https://doi.org/10.1023/A:1009266801160