Abstract
Novel homotetranuclear Cu(II) and heteronuclear Cu(II)-Mn(II) complexes with tetrathioether-tetrathiol moiety have been prepared and their DNA relaxation activities with plasmid pCYTEXP (5kb) were electrophoretically established. The cleavage products analyzed by neutral agarose gel electrophoresis indicated that the interaction of the metal complexes with supercoiled plasmid DNA yielded linear, nicked or degraded DNA. The relaxation activities of both homo- and heterotetranuclear (SK4) complexes are time- and concentration-dependent. The findings suggest that SK4 with potent nucleolytic activity is a good nuclease substitute in the presence of cooxidant. Furthermore, the observation of induction of DNA into smaller fragments by SK4 is also significant.
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Bajetta E, Rovej R, Buzzoni R, Vaglini M, Bonadonna G. 1982 Treatment of advanced malignant melanoma with vinblastine, bleomycin and cisplatin. Cancer Treat Rep 66, 1299-1302.
Barnard CFJ, Clear MJ, Hydes PC. 1987 Second generation of anticancer platinum compounds. Chem. Britain 22, 1001-1004.
Barton JK.1986 Metals and DNA: Molecular left-handed complements. Science 233, 727-733.
Belev TN, Singh M, McCarthy JEG. 1991 A fully modular vector system for the optimization of gene expression in Eschericia coli. Plasmid 26, 147-150.
Corey DR, Pei D, Schultz PG. 1990 Generation of a catalytic sequence-specific hybrid DNase. Biochemistry 28, 8277-8286.
Dervan PB. 1992 Reagents for the site-specific cleavage of megabase DNA. Nature 359, 87-88.
Dervan PB. 1986 Design of sequence-specific DNA-binding molecules. Science 232, 464-471.
Gravert DJ, Griffin JH. 1993 Specific DNA cleavage mediated by [salenMn(III)]C. J OrgChem 58, 820-822.
Groves JT, Farrell TP. 1989 DNA cleavage by a metal chelating tricationic porphyrin. J Am Chem Soc 111, 4998-5000.
Hertzberg RP, Dervan PB. 1984 Cleavage of DNA with methidium propyl-EDTA-iron(II): reaction conditions and product analyses. Biochemistry 23, 3934-3945.
Hertzberg RP, Dervan PB. 1982 Cleavage of double helical DNA by methidium propyl-EDTA-iron(II). J Am Chem Soc 104, 313-315.
Karaböcek S, Sengül H, Karaböcek N. 1999 Di-and tetranuclear copper(II) complexes of a novel binucleating tetrathioether-tetrathiol ligand. Transition Met Chem 24, 121-123.
Mandal SS, Kumar NV, Varshney U, Bhattacharya S. 1996 Metal-ion-dependent oxidative DNA cleavage by transition metal complexes of a new water soluble salen derivative. J Inorg Biochem 63, 265-272.
Meck MV, Lippard SJ. 1992 Unwinding of supercoiled DNA by platinium ethidium and related complexes. J Am Chem Soc 114, 3386-3389.
Meunier B. 1996 In DNA and RNA Cleavers, and Chemotherapy of Cancer and Viral Diseases, Dordrecht, Kluwer.
Nielsen PE. 1990 Chemical and photochemical probing of DNA complexes. J Mol Recog 3, 1-25.
Papavassiliou AG. 1995 Chemical nucleases as probes for studying DNA-protein interactions. Biochem J 305, 345-357.
Pope LE, Sigman DS. 1984 Secondary structure specificity of the nuclease activity of the 1,10-phenathroline-copper complexes. Proc Natl Acad Sci USA 81, 3-7.
Pratviel G, Duarte V, Bernadou J, Meunier B. 1993 Nonenzymatic cleavage and ligation of DNA at a three A.T base pair site: a two step pseudohydrolysis of DNA. J Am Chem Soc 115, 7939-7943.
Ross SA, Pittie M, Meunier B. 1999 Synthesis of two acridine conjugates of the bis(phenanthroline) ligand "Clip-Phen" and evaluation of the nuclease activity of the corresponding copper complexes. Eur J Inorg Chem 557-563.
Routier S, Bernier J-L, Waring MJ, Colson P, Houssier C, Bailly C. 1996 Synthesis of a functionalized salen-copper complex and its interaction with DNA. J OrgChem 61, 2326-2331.
Sargeson AM. 1996 The potential for the cage complexes in biology. Coord Chem Rev 151, 89-114.
Sato K, Chikira M, Fujii Y, Komatsu A. 1994 Stereospecific binding of chemically modified salen-type Schiff base complexes of copper(II) with DNA. J Chem Soc Chem Commun 5, 625-626.
Schultz PG, Taylor JS, Dervan PB. 1982 Design and synthesis of a sequence specific DNA cleaving molecule. J Am Chem Soc 104, 6861-6863.
Sherman SE, Lippard SJ. 1987 Structural aspects of platinum anti-cancer drug interactions with DNA. Chem Rev vn87, 1153-1181.
Sigman DS. 1986 Nuclease activity of 1,10-phenanthroline-copper ion. Acc Chem Res 19, 180-186.
Sigman DS. 1990 Chemical nucleases. Biochemistry 29, 9097-9105.
Sigman DS, Bruce TW, Mazumender A, Sutton CL. 1993 Targeted chemical nucleases. Acc Chem Res 26, 98-104.
Sigman DS, Graham DR, D'Aurura V, Stern AM. 1979 Oxygen-dependent cleavage of DNA by the 1,10-phenanthroline-cuprous complex. J BiolChem 254, 12269-12272.
Sigman DS, Mazumender A, Perrin DM. 1993 Chemical nucleases. Chem Rev 93, 2295-2316.
Sreedhara A, Patwardhan A, Cowan JA. 1999 Novel reagents for targeted cleavage of RNA sequences: towards a new family of inorganic pharmaceuticals. Chem Commun 1147-1148.
Travers AA. 1993 In: DNA-Protein Interactions, London: Chapman and Hall.
Tullius TD, Dombroski BA. 1986 Hydroxyl radical "footprinting": high-resolution information about DNA-protein contacts and application to lambda repressor and Cro protein. Proc Natl Acad Sci USA 83, 5469-5473.
Veal JM, Rill RL. 1988 Sequence specificity of DNA cleavage by bis(1,10-phenathroline)copper(I). Biochemistry 27, 1822-1827.
Ward B, Skorobogaty A, Dabrowiak JC. 1986 DNA cleavage specificity of a group of cationic metalloporphyrins. Biochemistry 25, 6875-6883.
Woodson SA, Muller JG, Burrows CJ, Rokita SE. 1993 A primer extension assay for modification of guanine by Ni(II) complexes. Nucleic Acid Res 21, 5524-5525.
Yoon C, Kuwabara MD, Law R, Wall R, Sigman DS. 1988 Sequence-dependent variability of DNA structure. Influence of flanking sequences and fragment length on digestion by conformationally sensitive nucleases. J Biol Chem 263, 8458-8463.
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Dülger, S., Saglam, N., Beldüz, A.O. et al. DNA cleavage by homo- and heterotetranuclear Cu(II) and Mn(II) complexes with tetrathioether-tetrathiol moiety. Biometals 13, 261–265 (2000). https://doi.org/10.1023/A:1009222705659
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DOI: https://doi.org/10.1023/A:1009222705659