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Stabilization of Micrococcus lysodeikticus cells towards lysis by lysozyme using glutaraldehyde: application as a novel biospecific ligand for the purification of lysozyme

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Biotechnology Techniques

Abstract

Micrococcus lysodeiekticus was stabilized against the lytic action of lysozyme by cross-linking with 5% (v/v) glutaraldehyde for 24 h but still retained its ability to bind lysozyme. An immobilized, biospecific ligand was prepared by covalently binding the cells to glutaraldehyde activated amino-Sepharose followed by stabilization of the cells with glutaraldehyde. Lysozyme bound specifically to this column and could be eluted by glycine/NaOH buffer (50 mM, pH 10.0) containing 2 M KCl.

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Authors and Affiliations

  1. Nuclear Agriculture and Biotechnology Division, Bhabha Atomic Research Centre, Trombay, Mumbai, 400085, India

    Stanislaus F. D'Souza & Kamalrookh Z. Marolia

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  1. Stanislaus F. D'Souza
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  2. Kamalrookh Z. Marolia
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D'Souza, S.F., Marolia, K.Z. Stabilization of Micrococcus lysodeikticus cells towards lysis by lysozyme using glutaraldehyde: application as a novel biospecific ligand for the purification of lysozyme. Biotechnology Techniques 13, 375–378 (1999). https://doi.org/10.1023/A:1008979709047

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  • DOI: https://doi.org/10.1023/A:1008979709047

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