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Protein separation using metal ion-bound particles in aqueous two-phase system

  • Published:
Biotechnology Techniques

Abstract

Metal ion affinity partitioning of protein in aqueous two-phase systems was studied using Sepharose as ligand carrier as an integrated adsorption partitioning. Cu(II)-bound Sepharose was mixed with protein solution and an aqueous two-phase system. The affinity sorbent was distributed quantitatively to the upper side or the interface. The binding studies of lysozyme to copper-bound gel in PEG/dextran two-phase systems demonstrate the feasibility of this bioseparation process. PEG/dextran system did not affect binding and elution of lysozyme to and from the Cu(II)-Sepharose particles.

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Authors and Affiliations

  1. Department of Food and Biotechnology, Kyonggi University, Suwon, 442–760, Korea

    Yun Joong Kwon

  2. Department of Biotechnology, Center for Chemistry and Chemical Engineering, und University, 22100 , Lund, Sweden

    Rajni Hatti-Kaul

Authors
  1. Yun Joong Kwon
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  2. Rajni Hatti-Kaul
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Joong Kwon, Y., Hatti-Kaul, R. Protein separation using metal ion-bound particles in aqueous two-phase system. Biotechnology Techniques 13, 145–148 (1999). https://doi.org/10.1023/A:1008943624956

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