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First protein affinity application of Cu2+-bound pure inorganic nanoflowers

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Abstract

Today, a new kind of materials is introduced to separation media day by day to increase the efficiency of the separation processes, and multiple-petalled nanostructured materials are one of them. In this study, new pure inorganic copper phosphate nanoflowers (pCP-NFs) were synthesized, and some environmental conditions affecting on binding mechanism with human serum albumin were evaluated via changing medium pH, temperature, initial human serum albumin (HSA) amount and salt concentrations. Before experimental studies, pCP-NFs were subjected to some characterization tests such as scanning electron microscopy, energy-dispersive X-ray, X-ray diffraction and Fourier transform infrared spectroscopy. Besides a lot of valuable instrumental data, some obtained experimental ones as follows: after Cu2+ ions attachment to pCP-NFs as ligand, maximum HSA adsorption capacity of obtained Cu2+-pCP-NFs was found as 225.7 mg/g with an initial concentration of 1.5 mg/mL at pH 7 and 25 °C. Langmuir and Freundlich adsorption equations were evaluated for determination of appropriate adsorption model in interaction, and Langmuir model found as the fittest one with a R2 of 0.9949 was also reviewed to determine Gibbs free energy between HSA and Cu2+-pCP-NFs interaction.

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Acknowledgements

This work was supported by The Scientific and Technological Research Council of Turkey (Grant Number:118Z037) and by Belarusian Republican Foundation for Fundamental Research, Grant B18TUB-001. Authors gratefully acknowledge use of the services and facilities of Technology Research and Implementation Center of Erciyes University (TAUM) and Scientific and Technological Application and Research Center of Aksaray University (ASUBTAM).

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Authors and Affiliations

  1. Biochemistry Division, Chemistry Department, Faculty of Arts and Science, Aksaray University, 68100, Aksaray, Turkey

    Burcu Önal & Mehmet Odabaşı

  2. Pharmacy Services Program, Vocational School of Health Science, Tarsus University, Tarsus, Turkey

    Ömür Acet

  3. Institute of Biophysics and Cell Engineering of the National Academy of Sciences of Belarus, Minsk, Belarus

    Volha Dzmitruk, Inessa Halets-Bui & Dzmitry Shcharbin

  4. Biochemistry Division, Chemistry Department, Faculty of Science, Erciyes University, 38030, Kayseri, Turkey

    Nalan Özdemir

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  1. Burcu Önal
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Önal, B., Acet, Ö., Dzmitruk, V. et al. First protein affinity application of Cu2+-bound pure inorganic nanoflowers. Polym. Bull. 79, 3233–3251 (2022). https://doi.org/10.1007/s00289-021-03557-5

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