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A colorimetric assay for gellan elaborated by Sphingomonas paucimobilis ATCC 31461

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Abstract

A colorimetric assay involving the dye toluidine blue O was developed to determine the concentration of the microbial heteropolysaccharide gellan elaborated by Sphingomonas paucimobilis ATCC 31461. Colour formation was linear up to a concentration of 0.7 mg/ml. The concentration of gellan produced in S. paucimobilis cultures was quantitated using this colorimetric dye-binding assay as well as the currently utilized gravimetric procedure, and comparable results were observed.

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Authors and Affiliations

  1. Olson Biochemistry Laboratories, Department of Chemistry and Biochemistry, South Dakota State University, Brookings, SD, 57007, USA

    T.P. West, B. Strohfus & M.F. Santiago

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  1. T.P. West
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  2. B. Strohfus
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  3. M.F. Santiago
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West, T., Strohfus, B. & Santiago, M. A colorimetric assay for gellan elaborated by Sphingomonas paucimobilis ATCC 31461. World Journal of Microbiology and Biotechnology 16, 529–531 (2000). https://doi.org/10.1023/A:1008935031777

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  • DOI: https://doi.org/10.1023/A:1008935031777

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