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Production of ligninolytic enzymes by Fusarium solani strains isolated from different substrata

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Abstract

A comparative study on the extracellular ligninolytic enzymatic activity of five strains of Fusarium solani in a carbon-limited medium under shaking, revealed a differential production of these enzymes. Aryl alcohol oxidase (AAO) activity was observed only in the supernatant of strain CLPS no. 568 with levels higher than 57 mU ml−1. Free extracellular laccase activity was detected in strains CLPS nos. 493, 568 and 570, strain no. 568 being the one which showed the highest activity (over 8.6 mU ml−1). Free extracellular lignin peroxidase (LiP) activity was not detected in any isolate tested, whereas low levels of manganese-dependent peroxidase (MnP) and manganese-independent peroxidase (MIP) activities were detected in certain isolates used. The AAO activity of F. solani on primary α-alcohols such as veratryl alcohol, is reported for the first time; this enzyme activity is hydrogen-peroxide independent. This is also the first report for extracellular MnP and MIP activities of F. solani.

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Authors and Affiliations

  1. Instituto de Botánica Spegazzini, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, 53 477, 1900, La Plata, Argentina

    Mario Carlos Nazareno Saparrat, Marta Noemí Cabello & Angélica Margarita Arambarri

  2. Unidad de Microbiología Aplicada, Centro de Investigaciones Biológicas, Consejo Superior de Investigaciones Científicas, Velázquez 144, 28006, Madrid, Spain

    María Jesús Martínez

  3. Cátedra de Farmacología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, 60 y 120, 1900, La Plata, Argentina

    Horacio Alfio Tournier

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  1. Mario Carlos Nazareno Saparrat
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  2. María Jesús Martínez
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  3. Horacio Alfio Tournier
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  4. Marta Noemí Cabello
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  5. Angélica Margarita Arambarri
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Saparrat, M.C.N., Martínez, M.J., Tournier, H.A. et al. Production of ligninolytic enzymes by Fusarium solani strains isolated from different substrata. World Journal of Microbiology and Biotechnology 16, 799–803 (2000). https://doi.org/10.1023/A:1008923804859

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