Skip to main content
SpringerLink
Log in

Simple gas chromatography method for lipase assay

  • Published:
Biotechnology Techniques

Abstract

A gas chromatography method for lipase assay using tributyrin as substrate is presented. Tributyrin is hydrolyzed by lipase to produce free butyric acid that is directly quantified by gas chromatography. The estimation of lipase activity takes only 6 min after enzyme reaction. The technique needs a small enzyme sample and is useful for analysis of large number of lipase samples. © Rapid Science Ltd. 1998

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Jaeger, K.E., Ransac, S., Dijkstra, B., Colson, C., Heuvel, M. and Misset, O. (1994). FEMS Microbiol. Rev. 15:29-63.

    Google Scholar 

  • McKellar, R. C. (1986). J. Dairy Res. 53:117-127.

    Google Scholar 

  • Omar, I.C., Nishio, N., and Nagai, S. (1987). Agric. Biol. Chem. 51:2153-2159.

    Google Scholar 

  • Vorderwülbecke, T., Kieslich, K. and Erdmann, H. (1992). Enzyme Microb. Technol. 14:631-639.

    Google Scholar 

  • Winkler, U.K. and Stuckmann, M. (1979). J. Bacteriol., 138:663-670.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Chemical Laboratory, Pune, India

    Neelima Kulkarni & R.V. Gadre

Authors
  1. Neelima Kulkarni
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R.V. Gadre
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Kulkarni, N., Gadre, R. Simple gas chromatography method for lipase assay. Biotechnology Techniques 12, 627–628 (1998). https://doi.org/10.1023/A:1008884130425

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008884130425

Keywords

Search

Navigation