Abstract
We describe the use of repetitive element sequence-based PCR (rep-PCR) on the two repetitive sequences, REP and ERIC elements, to distinguish members of closely related Salmonella species. Within the species, ERIC–PCR showed a higher discriminative potential than REP–PCR, but by using a combination of the two PCR methods it was possible to distinguish all the isolates examined. The rep-PCR fingerprints of Salmonella organisms were distinctly different from some Gram-positive bacteria, for example Staphylococcus, Bacillus megaterium, and even the closely related Escherichia coli and Serratia marcescens. Identical fingerprints were observed with whole-cell preparations. Rapid specimen preparation has enhanced the value of rep-PCR in timely analysis of epidemiological relationships.
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Koh-Luar, S., Choo, G., Tan, S. et al. Genomic fingerprints of Salmonella species generated with repetitive element sequence-based PCR. World Journal of Microbiology and Biotechnology 14, 17–22 (1997). https://doi.org/10.1023/A:1008860013816
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DOI: https://doi.org/10.1023/A:1008860013816