Abstract
A simple and efficient protocol involving the use of cetyltrimethylammonium bromide (CTAB) for rapid isolation of high molecular wt (≥ 50 kb) DNA from Streptomyces aureofaciens is described. The DNA yields range from 1.5–2.5 mg per 1.0 g (wet wt) of mycelia, with the purity measured at A260/280 of 1.83–1.97 and also at A260/230 of 2.2–2.71. The DNA preparation is suitable as substrate for restriction digestion, Southern hybridization and library construction. © Rapid Science Ltd. 1998
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Tripathi, G., Rawal, S. Simple and efficient protocol for isolation of high molecular weight DNA from Streptomyces aureofaciens. Biotechnology Techniques 12, 629–631 (1998). https://doi.org/10.1023/A:1008836214495
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DOI: https://doi.org/10.1023/A:1008836214495