Abstract
The objective of this research was to study the levels of some organic solutes, such as total protein, total soluble sugars, starch and proline in leaves, as well as abscisic acid concentration in xylem of pepper plants inoculated with Verticillium dahliae Kleb. Healthy and inoculated plants were always kept well watered. Measurements were made at time intervals after inoculation. Leaf water potential rapidly decreased as a consequence of fungal infection. However, relative water content in leaves only changed significantly from day 20 after inoculation, and such decreases coincided with a sharp build up of proline and total soluble sugars in leaves. Starch and protein levels, as well as abscisic acid concentration in xylem, declined in healthy and inoculated peppers as they became older. However, such decreases were more pronounced in infected plants, especially soon after inoculation. Results suggest that proline and total soluble sugars accumulation could be sensors of the damage caused by the fungal infection.
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Aguirreolea J, Irigoyen J, Sánchez-Díaz M and Salaverri J (1995) Physiological alterations in pepper during wilt induced by Phytophthora capsici and soil water deficit. Plant Pathol 44: 587–596
Aspinall D and Paleg LG (1981) Proline accumulation. Physiological aspects. In: Paleg LG and Aspinall D (ed) Physiology and Biochemistry of Drought Resistance in Plants (pp 205–240) Academic Press, New York
Beckman CH (1987) The nature of wilt diseases of plants. The American Phytopathological Society. Minnesota, USA
Bennet-Clark IA (1959) Water relations of cells. In: Steward FC (ed) Plant Physiology. Vol 2 (pp 105–191) Academic Press, New York
Bradford MM (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein dye-binding. Anal Biochem 72: 248–254
Cenoz S, García-Mina JM, Aguirreolea J and Jordana R (1997) Efecto de un sistema orgánico de origen natural sobre el desarrollo de plantas de pimiento afectadas por Verticillium dahliae. Phytoma 92: 122–124
Cooper RM and Wood RK (1980) Cell wall degrading enzymes of vascular wilt fungi. III. Possible involvement of endo-pectin lyase in Verticillium wilt of tomato. Physiol Plant Pathol 16: 285–300
Davies WJ, Tardieu F and Trejo CL (1994) How do chemical signals work in plants that grow in drying soil? Plant Physiol 104: 309–314
Dunn RN, Hedden P and Bailey JA (1990) A physiologically-induced resistance of Phaseolus vulgaris to a compatible race of Colletrotrichum lindemuthianum is associated with increases in ABA content. Physiol Mol Plant Pathol 36: 339–349
Fraser RSS (1991) ABA and plant responses to pathogens. In: Davies WJ and Jones HG (eds) Abscisic acid. Physiology and biochemistry (pp 189–200) Bios Scientific Publishers, Oxford, UK
Geigenberger P, Reimholz R, Geiger M, Merlo L, Canale V and Stitt M (1997) Regulation of sucrose and starch metabolism in potato tubers in response to short-term water deficit. Planta 201: 502–518
Goicoechea N, Antolín MC and Sánchez-Díaz M (1997) Gas exchange is related to the hormone balance in mycorrhizal or nitrogen-fixing alfalfa subjected to drought. Physiol Plantarum 100: 989–997
Hammerschmidt R (1984) Rapid deposition of lignin in potato tuber tissue as a response to fungi non-pathogenic on potato. Physiol Plant Pathol 24: 33–42
Hoyos GP, Laurer FI and Anderson NA (1993) Early detection of Verticillium wilt resistance in a potato breeding program. Am Potato J 70: 535–541
Irigoyen J, Emerich DW and Sánchez-Díaz M (1992) Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiol Plantarum 84: 55–60
Ismail MR and Davies WJ (1997) Water relations of Capsicum genotypes under water stress. Biol Plantarum 39: 293–297
Jarvis CE and Walker JRL (1993) Simultaneous, rapid, spectrophotometric determination of total starch, amylose and amylopectin. J Sci Food Agr 63: 53–57
Levin I (1984) Effect of phytohormones on the rate of development of brown rust on isolated leaves of wheat. Fiziol Rastenii 31: 356–361
Lorenzini G, Guidi L, Nali C, Ciompi S and Soldatini GF (1997) Photosynthetic response of tomato plants to vascular wilt diseases. Plant Sci 124: 143–152
Moore PH and Cosgrove DJ (1991) Developmental changes in cell and tissue water relations parameters in storage parenchyma of sugarcane. Plant Physiol 96: 794–801
Pegg GF (1989) Pathogenesis in vascular diseases of plants. In: Ayres PG (ed) Effects of Disease on the Physiology of the Growing Plant (pp 149–177) Cambridge University Press, Cambridge
Sánchez-Díaz M and Kramer PJ (1971) Behavior of corn and sorghum under water stress and during recovery. Plant Physiol 48: 613–616
Scholander PF, Hammel HT, Badstreet ED and Hemmingsen EA (1965) Sap pressure in vascular plants. Science 148: 339–346
Slatyer RO (1967) Plant-Water Relationships. Academic Press, New York
Tzeng DD and De Vay JE (1985) Physiological responses of Gossypium hirsutum L. to infection by defoliating and non-defoliating pathotypes of Verticillium dahliae Kleb. Physiol Plant Pathol 26: 57–72
Tzeng DD, Wakeman RJ and DeVay JE (1985) Relationships among Verticillium wilt development, leaf water potential, phenology, and lint yield in cotton. Physiol Plant Pathol 26: 73–81
Ward EWB, Cahill DM and Bhatacharyya MK (1989) Abscisic acid suppression of phenylalanine-ammonia-lyase activity and mRNA, and resistance of soybeans to Phytophtora megasperma f. sp. glycinea. Plant Physiol 91: 23–27
Weatherley PE (1950) Studies in the water relations of the cotton plant. I. The field measurements of water deficits in leaves. New Phytol 49: 81–87
Yemm EW and Willis AJ (1954) The estimation of carbohydrates in plant extracts by anthrone. Biochem J 57: 508–514
Zhang J and Davies WJ (1989) Abscisic acid produced in dehydrating roots may enable the plant to measure the water status of the soil. Plant Cell Environ 12: 73–81
Zhang B and Archbold DD (1993) Solute accumulation in leaves of a Fragaria chiloensis and a F. virginiana selection responds to water deficit stress. J Am Soc Hortic Sci 118: 280–285
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Goicoechea, N., Aguirreolea, J., Cenoz, S. et al. Verticillium dahliae Modifies the Concentrations of Proline, Soluble Sugars, Starch, Soluble Protein and Abscisic Acid in Pepper Plants. European Journal of Plant Pathology 106, 19–25 (2000). https://doi.org/10.1023/A:1008724816041
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DOI: https://doi.org/10.1023/A:1008724816041