Abstract
Electrophoretic analysis of seed albumins covered 105 Lathyrus sativus accessions originating from different geographical regions. Four major albumin bands were responsible for the detected variation. Due to differences in the number and combination of these bands, eleven electrophoretic seed albumin patterns were distinguished; four of them were rare phenotypes found in less than 1% of the 817 examined individuals. Electrophoretic data were subjected to statistical analysis using hierarchical UPGMA grouping and biplot techniques. Generally, no correlation was found between occurrence of either the seed albumin patterns or the major albumin bands and geographical origin of the forms under study. On the other hand, data concerning frequency distribution of the major albumin bands tended to separate two groups of accessions, white-seeded with large seeds and coloured-seeded with relatively small seeds; coloured-seeded accessions with rather large seeds mixed with the above groups.
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Campbell, C.G., R.B. Mehra, S.K. Agrawal, Y.Z. Chen, A.M. Abd El Moneim, H.I.T. Khawaja, C.R. Yadov, J.U. Tay & W.A. Araya, 1994. Current status and future strategy in breeding grasspea (Lathyrus sativus). Euphytica 73: 167–175.
Davis, B.J., 1964. Disc electrophoresis-II. Method and applications to human serum proteins. Ann. New York Acad. Sci. 121: 404–427.
Gabriel, K.R., 1971. The biplot graphic display of matrices with application to principal component analysis. Biometrika 58: 453–467.
Hanelt, P., 1972. Die infraspezifische Variabilität von Vicia faba L. und ihre Gliederung. Kulturpflanze 20: 75–128.
Harlan, J.R. & J.M.J. de Wet, 1971. Toward a rational classification of cultivated plants. Taxon 20: 509–517.
Jackson, M.T. & A.G. Yunus, 1984. Variation in the grasspea (Lathyrus sativus L.) and wild species. Euphytica 33: 549–559.
Kearney, J. & J. Smartt, 1995. The Grasspea Lathyrus sativus (Leguminosae-Papilionoideae). In: Smartt J. & N.W. Simmonds (Eds), Evolution of crop plants, pp. 266–270, Longmann, London.
Przybylska, J., 1986. Identification and classification of the Pisum genetic resources with the use of electrophoretic protein analysis. Seed Sci. & Technol. 14: 529–543.
Przybylska, J., 1995. Some examples of the use of electrophoretic protein analysis in taxonomic investigations of leguminous plants. J. Appl. Genet. 36: 255–271.
Przybylska, J. & Z. Zimniak-Przybylska, 1993. Electrophoretic seed albumin patterns in the cultivated Phaseolus species: albumin variants with trypsin inhibitory activity. Genet. Pol. 34: 15–25.
Przybylska, J. & Z. Zimniak-Przybylska, 1995. Electrophoretic seed albumin patterns and species relationships in Vicia sect. Faba (Fabaceae). Pl. Syst. Evol. 198: 179–194.
Przybylska, J. & Z. Zimniak-Przybylska, 1997. Electrophoretic seed albumin patterns in Vicia species of sections Hypechusa and Peregrinae (Fabaceae). Pl. Syst. Evol. 208: 239–248.
Przybylska, J., S. Blixt, J. Hurich & Z. Zimniak-Przybylska, 1977. Comparative study of seed proteins in the genus Pisum. I. Electrophoretic patterns of different protein fractions. Genet. Pol. 18: 27–38.
Singh, K.B. & R.S. Malhotra, 1984. Collection and evaluation of chickpea genetic resources. In: Witcombe, J.R. & W. Erskine (Eds), Genetic resources and their exploitation-chickpeas, faba beans and lentils, pp. 105–122, The Hague, Boston, Lancaster: Nijhoff/Junk.
Smartt, J., 1984. Evolution of grain legumes. I. Mediterranean pulses. Expl. Agric. 20: 275–296.
Smartt, J., 1990. Pulses of the classical world. In: Grain legumes. Evolution and genetic resources, pp. 176–244, Cambridge University Press.
Smartt, J., A. Kaul, W.M. Araya, M.M. Rahman & J. Kearney, 1994. Grasspea (Lathyrus sativus L.) as a potentially safe legume food crop. In: Muehlbauer F.J. & W.J. Kaiser (Eds), Expanding the production and use of cool season food legumes, pp. 144–155, Kluwer Academic Publishers, Dordrecht, The Netherlands.
Solh, M. & W. Erskine, 1984. Genetic resources of lentils. In: Witcombe, J.R. & W. Erskine (Eds), Genetic resources and their exploitation-chickpeas, faba beans and lentils, pp. 205–224, The Hague, Boston, Lancaster: Nijhoff/Junk.
Townsend, C.C. & E. Guest, 1974. Flora of Iraq, volume 3. Leguminales. Ministry of Agriculture and Agrarian Reform, Baghdad. Cited after: Smartt (1984).
Yunus, A.G., M.T. Jackson & J.P. Catty, 1991. Phenotypic polymorphism of six enzymes in the grasspea (Lathyrus sativus L.). Euphytica 55: 33–42.
Zimniak-Przybylska, Z. & J. Przybylska, 1994. Electrophoretic seed albumin patterns in wild and cultivated forms of Phaseolus vulgaris L. Genet.Pol. 35: 171–182.
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Przybylska, J., Zimniak-Przybylska, Z. & Krajewski, P. Diversity of seed albumins in the grasspea (Lathyrus sativus L.): an electrophoretic study. Genetic Resources and Crop Evolution 45, 423–430 (1998). https://doi.org/10.1023/A:1008668908359
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DOI: https://doi.org/10.1023/A:1008668908359