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Influence of chlorophyll concentrations on the reliability of measurements with an oxygen electrode when studying photosynthetic efficiency in bioassays using microalgae

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Abstract

Euglena gracilis and Chlamydomonas reinhardtii were used as model organisms to establish the best conditions for studying photosynthetic efficiency using the Light Pipette – experimental system, which enables sensitive detection of changes in oxygen evolution/consumption and an easy collection and digitalisation of data. Chlorophyll concentrations of 0.005, 0.025, 0.050 and 0.075 mgmL-1 were investigated using different light regimes. Cultures of E. gracilis at the same chlorophyll concentration absorbed more light(measured at 580 μmol m-2 s-1) than those of C. reinhardtii. Cell density had a considerable effect on the reliability of measurements. Chlorophyll concentrations between 0.025 mgChl ml-1 and 0.050 mgChlml-1 can be recommended when applying the Light Pipette system in bioassays using microalgae.

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Authors and Affiliations

  1. Department of Applied Science, Mid Sweden University, 871 88, Härnösand, Sweden

    Roman A. Danilov & Nils G.A. Ekelund

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  1. Roman A. Danilov
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  2. Nils G.A. Ekelund
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Danilov, R.A., Ekelund, N.G. Influence of chlorophyll concentrations on the reliability of measurements with an oxygen electrode when studying photosynthetic efficiency in bioassays using microalgae. Journal of Applied Phycology 12, 63–67 (2000). https://doi.org/10.1023/A:1008189230953

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