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Improved sensitivity of trypan blue dye exclusion assay with Ni2+ or Co2+ salts

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Abstract

A modified trypan blue dye exclusion assay was developed usingNi2+ or Co2+ salts to determine the viability ofprimary and transformed cells. When the cells were preincubatedwith NiSO4 or CoCl2 followed by trypan blue assay, thecontrast between stained and unstained cells was significantlyincreased as compared to the conventional trypan blue dyeexclusion assay.

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Authors and Affiliations

  1. Department of Organic Chemistry and Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560 012, India

    K. D. Sarma

  2. Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560 012, India

    D. Ray

  3. Department of Microbiology and Cell Biology, Indian Institute of Science, Bangalore, 560 012, India

    A. Antony

Authors
  1. K. D. Sarma
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  2. D. Ray
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  3. A. Antony
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Sarma, K.D., Ray, D. & Antony, A. Improved sensitivity of trypan blue dye exclusion assay with Ni2+ or Co2+ salts. Cytotechnology 32, 93–95 (2000). https://doi.org/10.1023/A:1008144527206

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  • DOI: https://doi.org/10.1023/A:1008144527206

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