Abstract
STREAMLINE Phenyl is a new hydrophobic interaction chromatography support designed for use in expanded bed adsorption. The phenyl groups are linked to STREAMLINE matrix via highly stable ether linkages. Within this development project the chemical and chromatographic stability as well as the breakthrough capacity for human IgG has been studied. The chemical stability was monitored as the carbon leakage from the matrix to the storage solution, pH 1–14 at 20 and 40 °C. The carbon content in the supernatant was determined with Total Organic Carbon (TOC) technique. In the chromatographic stability study STREAMLINE Phenyl was stored in eight different storage solutions under ambient conditions for 12 weeks and then tested in a chromatographic function test. The results show that the adsorbent is chemically stable and that the chromatographic properties are retained under the tested conditions. The breakthrough capacity study demonstrates the importance of the bed height for obtaining maximal dynamic capacity. Further, there is a good correlation between breakthrough data generated from packed bed and expanded bed runs.
Similar content being viewed by others
References
Chase HA (1994) Purification of proteins by adsorption chromatography in expanded beds. Trends Biotechnol. 12: 296–303.
Hjorth R (1997) Expanded-bed adsorption in industrial bioprocessing: recent developments. Trends Biotechnol. 15: 230–235.
Jansson J-C and Rydén L (1998) Protein Purification Principles, High Resolution Methods, and Applications, 2nd edition. John Wiley and Sons, Inc., New York.
Thömmes J (1997) Fluidized bed adsorption as a primary recovery step in protein purification. In: T. Scheper (ed.), Advances in Biochemical Engineering/Biotechnology, Vol. 58 (pp.185–230). Springer-Verlag, Berlin.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Färenmark, J., Gustavsson, J., Lagerlund, I. et al. Characterisation of STREAMLINETM Phenyl. Bioseparation 8, 139–144 (1999). https://doi.org/10.1023/A:1008106712159
Issue Date:
DOI: https://doi.org/10.1023/A:1008106712159