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Globulin and albumin proteins in dehulled seeds of three Phaseolus vulgaris cultivars

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Abstract

Albumin and globulin fractions, obtained after modifications of the Osborne's fractionation scheme, were investigated in the seeds of three cultivars of Phaseolus vulgaris, after removal of the seed coats, to prevent interactions of tannins and phenols with the globulins. Also, by using an appropriate buffer to separate the globulins more efficiently, no cross-contaminations were observed in SDS-PAGE between these two fractions. The combination of these two procedures resulted in globulin to albumin ratios 70percnt higher than those published previously with the same cultivars. Amino acid compositions of total globulin, phaseolin and albumin fractions are presented. Whereas the albumins contained much more cysteine than the globulins, the concentrations of methionine were evenly distributed in these two fractions. Also, the bulk of methionine was found in phaseolin; cysteine was found in non-phaseolin proteins. In addition, the globulins contained unexpectedly high amounts of lysine.

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References

  1. George AA, Lumen BO, Whitaker JR, Sgarbieri VC (1993) Methionine-containing proteins in two Phaseolus vulgariscultivars with different methionine bioavailabilities. Plant Foods Human Nutr 43: 225-232.

    Article  CAS  Google Scholar 

  2. Durigan JF, Sgarbieri VC, Bulisani A (1987) Protein value of dry bean cultivars: Factors interfering with biological utilization. J Agric Food Chem 35: 694-698.

    Article  CAS  Google Scholar 

  3. Deshpande SS, Sathe SK, Salunkhe DK (1984) Chemistry and safety of plant polyphenols. In Friedman M (ed), Nutritional and toxicological aspects of food safety. Plenum Press, New York.

    Google Scholar 

  4. Deshpande SS, Nielsen SS (1987) Nitrogenous constituents of selected grain legumes. J Food Sci 52: 1321-1325.

    Article  CAS  Google Scholar 

  5. Mossé J, Pernollet JC (1983) Storage proteins of legume seeds. In Arora SK (ed), Chemistry and biochemistry of legumes. Oxford and IBH Publishing Co., New Delhi, pp. 111–193.

    Google Scholar 

  6. Gepts, Bliss FA (1984) Enhanced available methionine concentration associated with higher phaseolin levels in common bean seeds. Theor Appl Genet 69: 47-53.

    Article  CAS  Google Scholar 

  7. Osborne TB (1907) The proteins of the wheat kernels. In Judd and Detweiler, Carnegie Institute, Publ. No. 84, Washington DC.

  8. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72: 248-254.

    Article  CAS  Google Scholar 

  9. Pusztai A, Duncan I (1971) Glycoprotein I of Phaseolus vulgaris: Homogeneity and enzymatic properties. Biochem Biophys Acta 229: 785-794.

    CAS  Google Scholar 

  10. Pusztai A, Stewart JC (1978) Isolectins of Phaseolus vulgaris: Physicochemical studies. Biochem Biophys Acta. 536: 38-49.

    CAS  Google Scholar 

  11. Jarret HW, Cooksy KD, Ellis B, Anderson JM (1986) The separation of ophthaldialdehyde derivatives of amino acids by reversed-phase chromatography on octylsilica column. Anal Biochem 153: 189-198.

    Article  Google Scholar 

  12. Näsholm T, Sandberg G, Ericsson A (1987) Quantitative analysis of amino acids in conifer tissues by high-performance liquid chromatography and fluorescence detection of their 9-fluorenylmethyl-chloroformate derivatives. J Chromatogr 396: 225-236.

    Article  Google Scholar 

  13. Moore S (1963) On the determination of cysteine as cysteic acid. J Biol Chem 238: 235-237.

    CAS  Google Scholar 

  14. Marur CJ, Sodek L, Magalhães ACN (1994) Free amino acids in leaves of cotton plants under water deficit. Rev Bras Fisiol Veg 6: 103-108.

    CAS  Google Scholar 

  15. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680-685.

    Article  CAS  Google Scholar 

  16. Stegemann H, Pietsch G. (1983) Methods for characterization of the seed proteins in cereals and legumes, Ch.3. In Gottschalk W, Müller HP (eds) Seed proteins: biochemistry, genetics, nutritive value. Martinus Nijhoff, The Hague.

    Google Scholar 

  17. Bhatty RS (1982) Albumin proteins of eight edible grain legume species: Electrophoretic patterns and amino acid composition. J Agric Food Chem 30: 620-624.

    Article  CAS  Google Scholar 

  18. Santoro LG (1988) Nutritional utilization by monogastric animal of glycoprotein-II (Phaseolin), the major 7S protein from kidney beans (Phaseolus vulgaris). In vivo and in vitro degradation of glycoprotein-II by rat intestinal proteases. PhD thesis, University of Aberdeen, The Rowett Research Institute, Aberdeen, UK.

  19. Chagas EP (1993) Identificação e caracterização parcial de albuminas e globulinas de três cultivares brasileiros de Phaseolus vulgaris.MSc thesis, Universidade Estadual de Campinas, São Paulo.

  20. Deshpande SS, Sathe SK, Salunkhe DK, Conforth DP (1982) Effects of dehulling on phytic acid, polyphenols and enzyme inhibitors of dry beans (Phaseolus vulgarisL.). J Food Sci 47: 1846-1850.

    Article  CAS  Google Scholar 

  21. Ma Y, Bliss FA (1978) Seed proteins of common bean. Crop Sci 17: 431-437.

    Article  Google Scholar 

  22. Pusztai A, Watt WB (1970) Glycoprotein-II. The isolation of a major antigenic and non haemagglutinating glycoprotein from Phaseolus vulgaris. Biochem Biophys Acta 207: 413-431.

    CAS  Google Scholar 

  23. Ishino K, Ortega MLD (1975) Fractionation and characterization of major reserve proteins from seeds of Phaseolus vulgaris. J Agric Food Chem 23: 529-533.

    Article  CAS  Google Scholar 

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Authors and Affiliations

  1. Dep. Fisiologia Vegetal, Universidade Estadual de Campinas, CEP 13083-970, Campinas, SP CP 6109, Brasil

    Eliana P. Chagas & Luiz G. Santoro

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  1. Eliana P. Chagas
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  2. Luiz G. Santoro
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Chagas, E.P., Santoro, L.G. Globulin and albumin proteins in dehulled seeds of three Phaseolus vulgaris cultivars. Plant Foods Hum Nutr 51, 17–26 (1997). https://doi.org/10.1023/A:1007971329420

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