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Concentrating Autographa californica nuclear polyhedrosis virus and recombinant alkaline phosphatase from insect cells using a temperature-sensitive hydrogel

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Abstract

Recombinant alkaline phosphatase expressed in insect cells was concentrated by a factor of one and half times at a separation efficiency of 54.2% using hydrogel ultrafiltration. Enzyme concentration was confirmed by SDS-PAGE as well as by spectrophotometric measurement. Wild and recombinant Autographa californica nuclear polyhedrosis viruses (AcNPV) were concentrated 1.4 and 1.6 times of the feed solution at 48.5 and 60.0% separation efficiency, respectively. Hydrogel ultrafiltration appears to be an attractive alternative for the concentration of AcNPV and recombinant proteins from insect cells.

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References

  • Arora DJS, Tremblay P, Bourgault R and Boileau S (1985) Concentration and purification of influenza virus from allantoic fluid. Anal. Biochem. 144: 189–192.

    Article  PubMed  CAS  Google Scholar 

  • Cheryan M (1986) Ultrafiltration Handbook. Technomic Publishing Co., Inc., Lancaster, Pennsylvania.

    Google Scholar 

  • Chung IS and Shuler ML (1993) Effect of Trichoplusia ni BTITn 5BI-4 cell density on human secreted alkaline phosphatase production. Biotechnol. Lett. 15: 1007–1012.

    Article  CAS  Google Scholar 

  • Davis TR, Wickham TY, McKenna KA, Grandos RR, Shuler ML and Wood HA (1992) Comparative recombinant protein production of eight insect cell lines. In Vitro Cell. Dev. Biol. 29A, 388–390.

    Google Scholar 

  • Freitas RFS and Cussler EL (1987) Temperature-sensitive gels as extraction solvents. Chem. Eng. Sci. 42: 97–103.

    Article  CAS  Google Scholar 

  • Freitas RFS (1986) Extraction with and phase behavior of temperature-sensitive gels. Ph.D. Thesis. University of Minnesota, Minneapolis, MN.

    Google Scholar 

  • Han J, Park CH and Ruan R (1995) Concentrating alkaline serine protease, subtilisin using a temperature-sensitive hydrogel. Biotechnol. Lett. 17: 851–852.

    Article  CAS  Google Scholar 

  • Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685.

    Article  PubMed  CAS  Google Scholar 

  • Park CH and Orozco-Avila I (1992) Concentrating cellulases from fermented broth using temperature-sensitive hydrogel. Biotech. Prog. 8: 521–526.

    Article  CAS  Google Scholar 

  • Park CH and Orozco-Avila I (1993) Concentrating cellulases using a temperature-sensitive hydrogel: effect of hydrogel particle size and geometry. Biotech. Prog. 9: 640–646.

    Article  CAS  Google Scholar 

  • Reed L and Muench H (1938) A simple method for estimating fifty percent endpoint. Am. J. Hyg. 27: 493–497.

    Google Scholar 

  • Trank SJ, Johnson DW and Cussler EL (1989) Isolated soy protein production using temperature-sensitive gels. Food Technol. 43: 78–83.

    CAS  Google Scholar 

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Authors
  1. Jong Hwa Park
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  2. Chang-Ho Park
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  3. In Sik Chung
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Park, J.H., Park, CH. & Chung, I.S. Concentrating Autographa californica nuclear polyhedrosis virus and recombinant alkaline phosphatase from insect cells using a temperature-sensitive hydrogel. Cytotechnology 25, 227–230 (1997). https://doi.org/10.1023/A:1007902119501

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  • DOI: https://doi.org/10.1023/A:1007902119501

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