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Intracoronary Administration of Adenosine Triphosphate Increases Coronary Blood Flow and Attenuates the Severity of Myocardial Ischemic Injury in Dogs

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Abstract

ATP generates nitric oxide (NO) via activation of P2y receptors, and is degraded to adenosine. This study was undertaken to examine whether ATP causes coronary hyperemic flow via purinoceptors-, NO- and adenosine-dependent mechanisms, and attenuates the severity of contractile and metabolic dysfunction in the ischemic myocardium. In the non-ischemic canine hearts, the infusions of ATP into the coronary artery dose-dependently increased coronary blood flow. The levels of adenosine and end-product of NO in coronary venous blood over the arterial blood also increased. This hyperemic flow was partially attenuated by either 8-sulfophenyltheophylline (8SPT) or Lο-nitro arginine methyl ester (L-NAME), and completely blocked by the treatment with 8SPT, L-NAME and suramin (SRM). During myocardial ischemia, exogenous ATP increased coronary blood flow, and attenuated myocardial metabolic and contractile dysfunction, which was completely blunted by the treatment with 8SPT, L-NAME and SRM. We conclude that exogenous ATP increases coronary blood flow in the non-ischemic and ischemic myocardium mainly via either NO- or adenosine-dependent mechanisms.

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Authors and Affiliations

  1. Department of Internal Medicine and Therapeutics, Osaka University Graduate School of Medicine, Suita

    Masafumi Kitakaze, Koichi Node, Kazuo Komamura, Tetsuo Minamino, Tsunehiko Kuzuya & Masatsugu Hori

  2. Department of Physiology I, Osaka University Graduate School of Medicine, Suita, Japan

    Hiroaki Kosaka

Authors
  1. Masafumi Kitakaze
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  2. Koichi Node
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  3. Kazuo Komamura
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  4. Tetsuo Minamino
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  5. Hiroaki Kosaka
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  6. Tsunehiko Kuzuya
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  7. Masatsugu Hori
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Kitakaze, M., Node, K., Komamura, K. et al. Intracoronary Administration of Adenosine Triphosphate Increases Coronary Blood Flow and Attenuates the Severity of Myocardial Ischemic Injury in Dogs. Cardiovasc Drugs Ther 13, 407–414 (1999). https://doi.org/10.1023/A:1007899822136

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