Abstract
The potyviruses, PVY (potato virus Y) and PVMV (pepper veinal mottle virus) were identified by serological and symptom diagnosis in pepper (Capsicum annuum L.) in Tunisia during 1995–1997. Both were known to be major constraints to pepper production in North Africa. Pepper veinal mottle virus (PVMV) has not been reported previously in North Africa, but PVMV was found to be widely spread in peppers throughout Tunisia. By using reverse transcription followed by polymerase chain reaction (RT-PCR), we have now amplified the PVMV coat protein gene of two Tunisian isolates. DNA polymorphism, determined by restriction fragment length polymorphism (RFLP) of the coat protein gene, revealed two patterns distinguishing PVMV from PVY. Within PVMV isolates, molecular and biological properties showed no polymorphism suggesting that all isolates are representative of one PVMV strain occurring in Tunisia.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Agranovsky AA (1993) Virus diseases of pepper (Capsicum annuum L.) in Ethiopia. J Phytopathol 138: 89–97.
Brunt AA, Kenten RH (1971) Pepper veinal mottle-a new member of the potato virus Y group from peppers (Capsicum annuum L. and C. fructescens L.) in Ghana. Ann Appl Biol 69: 235–243.
Brunt AA, Kenten RH (1972) Pepper veinal mottle virus. CMI/AAB Descriptions of Plant Viruses No. 104, 4 pp.
Brunt AA, Kenten RH, Phillips S (1978) Symptomatologically distinct strains of pepper veinal mottle virus from four West African solanaceous crops. Ann Appl Biol 88: 115–119.
Clark MF, Adams AN (1977) Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. J Gen Virology 34: 574–586.
Conti M, Masenga V (1977) Identification and prevalence of pepper viruses in Northwest Italy. Phytopathology 90: 212–222.
Conti M, Marte M (1983) Virosi e micoplasmosi del pepperone. Estratto da l'Italia Agricola 120: 132–152.
de Wijs JJ (1973) Pepper veinal mottle virus in Ivory Coast. Neth J Plant Pathol 79: 189–193.
Dougherty WG, Hiebert E (1980) Translation of potyvirus RNA in a rabbit reticulocyte lysate: cell-free translation strategy and a genetic map of the potyviral genome. Virology 104: 183–194.
Fakhfakh H, Makni M, Khadmaoui A, Cherif C, Elgaaied A, Marrakchi M (1994) Le virus Y de la pomme de terre dans les cultures de piment en Tunisie: Identification et caractérisation des souches par voie biologique, préparation d'anticorps polyclonaux anti-PVY. Biologia 1: 52–69.
Fakhfakh H, Makni M, Robaglia C, Elgaaied A, Marrakchi M (1995) Polymorphisme des régions capside et 30NTR de 3 isolats tunisiens du virus Y de la pomme de terre (PVY). Agronomie 15: 569–579.
Gebré-Selassié K, Marchoux G, Pochard E (1983) Biological and serological characterization of potato virus Y strains affecting peppers and other related strains. Capsicum Newsletter 2: 134–136.
Gebré-Selassié K, Marchoux G, Delecolle B and Pochard E (1985) Variabilité naturelle des souches du virus Y de la pomme de terre dans les cultures de piment du Sud-Est de la France. Caractérisation, classification en pathotypes. Agronomie 5: 621–630.
Gubler U, Hoffman BJ (1983) A simple and very efficient method for generating cDNA libraries. Gene 25: 263–269.
Huguenot C, Furneaux MT, Clare J, Hamilton RI (1996) Serodiagnosis of pepper veinal mottle virus in West Africa using specific monoclonal antibodies in DAS-ELISA. J Phytopathol 144: 29–32.
Koenig R, Paul HL (1982) Variants of ELISA in plant virus diagnosis. J Virol Meth 5: 113–125.
Marchoux G, Gebré-Selassié K, Quiot JB(1976) Observations préliminaires concernant les souches et les plantes réservoirs du virus Y de la pomme de terre dans le Sud-Est de la France. 4è me Congrè s upm zadar (Yougoslavie). Agric Conspect Sci 39: 541–552.
Marchoux G, Delecolle B, Gebré-Selassié K (1993) Systemic infection of tobacco by pepper veinal mottle potyvirus (PVMV) depends on the presence of potato virus Y (PVY). J Phytopathol 137: 283–292.
Nelson MR, Wheeler RE (1972) A new virus disease of pepper in Arizona. Plant Dis 56: 731–735.
Robaglia C, Durand-Tardif M, Tronchet M, Boudazin G, Astier-Manifacier S, Casse-Delbart F (1989) Nucleotide sequence of potato virus Y (strain N). J G Virol 70: 935–947.
Southern EM (1975) Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol 98: 503–517.
Ullrich A, Shine J, Chirgwin J, Pictet R, Tischer E, Rutter WJ, Goodman HM (1977) Rat insulin genes: construction of plasmids containing the coding sequences. Science 196: 1313–1319.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Gorsane, F., Fakhfakh, H., Tourneur, C. et al. Some Biological and Molecular Properties of Pepper Veinal Mottle Virus Isolates Occurring in Tunisia. Plant Molecular Biology Reporter 17, 149–158 (1999). https://doi.org/10.1023/A:1007560222288
Issue Date:
DOI: https://doi.org/10.1023/A:1007560222288