Abstract
Purpose. The aim of the study was to investigate whetherdopamine is transferred along the olfactory pathway to the brain followingnasal administration to mice.
Methods. [3H]-Dopamine was administered nasally or intravenouslyto female mice. Brain tissue samples were excised and the radioactivecontent was measured. The precise localisation of dopamineradioactivity in the brain was studied using autoradiography. The presence ofdopamine or its metabolites in the olfactory bulb and mucosa wasascertained using thin layer chromatography (TLC).
Results. After administration of [3H]-dopamine into the rightnostril, the amount of dopamine in the right bulb increased with time until,after 4 h, it was 27 times higher than in the left bulb. Among the otherbrain tissue samples, significantly higher amount of radioactivity wasdetected in the lateral olfactory tract. Radioactivity in the right olfactorybulb was shown by autoradiography to be selectively located in theperipheral layers 1 to 4 h after administration. Selective uptake ofradioactivity was not seen in other regions of the brain. TLC dataindicated that approximately 75% and 10% of the radioactivity in theolfactory bulb and mucosa, respectively, coeluted with dopamine.
Conclusions. The results indicate that unchanged dopamine istransferred into the olfactory bulb following nasal administration of[3H]-dopamine.
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Dahlin, M., Bergman, U., Jansson, B. et al. Transfer of Dopamine in the Olfactory Pathway Following Nasal Administration in Mice. Pharm Res 17, 737–742 (2000). https://doi.org/10.1023/A:1007542618378
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DOI: https://doi.org/10.1023/A:1007542618378