Abstract
Simultaneous presence of two chaperones, GroEL and protein disulfide isomerase (PDI), assists the reactivation of denatured D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in an additive way. Delayed addition of chaperones to the refolding solution after dilution of denatured GAPDH indicates an interaction with intermediates formed mainly in the first 5 min for PDI and formed within a longer time period for GroEL-ATP. The above indicate that the two chaperones interact with different folding intermediates of GAPDH. After delayed addition of one chaperone to the refolding mixture containing the other at 4°C, GroEL binds with all GAPDH intermediates dissociated from PDI, and PDI interacts with the intermediates released from GroEL during the first 10–20 min. It is suggested that the GAPDH folding intermediates released from the chaperone-bound complex are still partially folded so as to be rebound by the other chaperone. The above results clearly support the network model of GroEL and PDI.
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REFERENCES
Badcoe, I. G., Smith, C. J., Wood, S., Halsall, D. J., Holbrook, J. J., Lund, P., and Clarke, A. R. (1991). Biochemistry 30, 9191–9200.
Bradford, M. M. (1976). Anal. Biochem. 72, 248–254.
Cai, H., Wang, C. C., and Tsou, C. L. (1994). J. Biol. Chem. 269, 24550–24552.
Chen, J., Song, J. L., Zhang, S., Wang, Y., Cui, D. F., and Wang, C. C. (1999). J. Biol. Chem. 274, 19601–19605.
Chen, L. and Sigler P. B. (1999). Cell 87, 757–768.
Ellis, R. J. (1997). Curr. Biol. 7, R531–R533.
Ellis, R. J. (1999). Curr. Biol. 9, R137–R139.
Goldberg, M. S., Zhang, J., Sondek, S., Matthews, C. R., Fox, R. O., and Horwich, A. L. (1997). Proc. Natl. Acad. Sci. USA 94, 1080–1085.
Lambert, N. and Freedman, R. B. (1983). Biochem. J. 213, 225–234.
Landry, S. J. and Gierasch, L. M. (1991). Biochemistry 30, 7359–7362.
Li, J. and Wang, C. C. (1999). J. Biol. Chem. 274, 10790–10794.
Li, X. L., Lei, X. D., Cai, H., Li, J., Yang, S. L., Wang, C. C., and Tsou, C. L. (1998). Biochem. J. 331, 505–511.
Liang, S. J., Lin, Y. Z., Zhou, J. M., Tsou, C. L., Wu, P., and Zhou, Z. K. (1990). Biochim. Biophys. Acta 1038, 240–246.
Martin, J., Langer, T., Boteva, R., Schramel, A., Horwich, A. L., and Hartl, F. U. (1991). Nature 352, 36–42.
Muronetz, V. I., Zhang, N. X., Bulatnikov, I. G., and Wang, C. C. (1998). FEBS Lett. 426, 107–110.
Puig, A. and Gilbert, H. F. (1994). J. Biol. Chem. 269, 7764–7771.
Quan, H., Fan, G. B., and Wang, C. C. (1995). J. Biol. Chem. 270, 17078–17080.
Scheek, R. M. and Slater, E. C. (1982). Meth. Enzymol. 89, 305–308.
Schmidt, M., Rutkat, K., Rachel., Pfeifer, F., Jaenicke, R., Viitanen, P., Lorimer F., and Buchner, J. (1994). Science 265, 656–659.
Shao, F., Bader, M. W., Jakob, U., and Bardwell, J. C. A. (2000). J. Biol. Chem. 275, 13349–13352.
Shtilermam, M., Lorimer, G. H., and Englander, S. W. (1999). Science 264, 822–825.
Todd, M. J., Lorimer, G. H., and Thirumalai, D. (1996). Proc. Natl. Acad. Sci. USA 93, 4030–4035.
van den Berg, B., Ellis, R. J., and Dobson, C. M. (1999). EMBO J. 18, 6927–6933.
Wang, C. C. (1998). Biochemistry (Moscow) 63, 407–412.
Yao, Y., Zhou, Y. C., and Wang, C. C. (1997). EMBO J. 16, 651–658.
Zhang, N. X. and Wang, C. C. (1999). Eur. J. Biochem. 264, 1002–1008.
Zheng, W. D., Quan, H., Song, J. L., Yang, S. L., and Wang, C. C. (1997). Arch. Biochem. Biophys. 337, 326–331.
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Zhang, Nx., Li, J. & Wang, Cc. GroEL and Protein Disulfide Isomerase Each Binds with Folding Intermediates of D-Glyceraldehyde-3-Phosphate Dehydrogenase Released from Complexes Formed with the Other. J Protein Chem 19, 569–574 (2000). https://doi.org/10.1023/A:1007146217946
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DOI: https://doi.org/10.1023/A:1007146217946