Abstract
The production of a reagent kit has been recently organized by DAKO, Immunotekh and other companies, for phenotyping of lymphocytes by the streptavidin–biotin method. The method needs no sophisticated equipment, is highly sensitive, and allows rapid staining of different lymphocyte subpopulations in capillary blood smears and subsequent observation of them under a light microscope. We have modified this method for staining leukocytes in the monolayer prepared with a plate cytorotor. Not decreasing the above-mentioned advantages of the method, this modification significantly cheapens and simplifies the staining procedure; the blood cells of 16 subjects can be stained concurrently, and the staining can be performed by a technician. The streptavidin–biotin method of lymphocyte phenotyping can be mastered in every immunological laboratory, thus improving its technical level.
Similar content being viewed by others
REFERENCES
Stepanova, E.N., Diagnosis of the AIDS-Infection and Hepatitis, News Appl. Immunol. Allergol., 1999, no. 1(3), p. 6.
Lebedev, K.A. and Ponyakina, I.D., Immunogramma v klinicheskoi praktike (Immunogram in Clinical Practice), Moscow: Nauka, 1990.
Lebedev, K.A., Immunocytochemical Analysis, in Printsipy i metody tsitokhimicheskogo analiza (Principles and Approaches in Clinical Analysis), Moscow: Meditsina, 1971, p. 238.
Hayry, P.K., Totterman, T.H., and Ranki, A., Lack of Subclass Selection during Density Purification of Human Lymphocytes, Clin. Exp. Immunol., 1977, vol. 28, no. 2, p. 341.
DAKO A S 2000 Catalogue, Copenhagen, March, 2000.
DAKO Envision: A Major Breakthrough in Immunohistochemical Staining, Copenhagen, 1999.
Giorno, R., A Comparison of Two Immunoperoxidase Staining Methods Based on the Avidin-Biotin Interaction, Diagn. Immunol., 1984, vol. 2, p. 161.
Instructions DAKO LSAB-2 System, Peroxidase Universal, Copenhagen: DAKO Corporation, 2000, p. 34.
Kompleksnoe immunologicheskoe obsledovanie bol'nykh v stomatologocheskoi praktike. Metodicheskie rekomendatsii MZ RSFSR (Immunological Examination of Patients in Stomatology: Methodical Recommendations, Ministry of Health of the Russian Federation), Moscow, 1989.
Totolyan, A.A., Bodudieva, I.A., Bubenov, L.N., et al., Standardization of Methods for Phenotyping of Human Blood and Bone Marrow Cells, Med. Immunol., St. Petersburg, 1999, vol. 1, no. 5, p. 21.
Eremin, O. and Binns, R.M., Mouse Red Blood Cell Rosettes: Human B and Some T Lymphocytes Express Receptors for Mouse Erytrocytes in the Presence of Ficoll, Immunology, 1982, vol. 47, no. 2, p. 321.
Petrov, R.V., Lebedev, K.A., Simonova, A.E., et al., The Ratio of the T-Lymphocyte Subpopulations Revealed by Methods of Monoclonal Antibodies and Rosette-Forming, Fiziol. Chel., 1986, vol. 12, no. 1, p. 94.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Lebedev, K.A., Ponyakina, I.D. & Kozachenko, N.V. A New Stage in the Performance of a Human Immunogram: A Visual Streptavidin-Biotin Approach. Human Physiology 27, 122–126 (2001). https://doi.org/10.1023/A:1007127912441
Issue Date:
DOI: https://doi.org/10.1023/A:1007127912441