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A new technique distinguishing α2-3 sialyl linkage from α2-6 linkage in sialyllactoses and sialyl-N-acetyllactosamines by post-source decay fragmentation method of MALDI-TOF mass spectrometry

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Abstract

α2-3 and α2-6 sialyl linkage types of sialyllactoses and sialyl-N-acetyllactosamines were analyzed by post-source decay (PSD) fragmentation method using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. A new matrix of norharmane was suited for the MALDI-TOF measurements of sialyl oligosaccharides. The fragment ions B1 produced by the cleavage of α2-3 sialyl linkages indicate much higher intensity than those produced by the cleavage of α2-6 sialyl linkages in sialyllactoses and sialyl-N-acetyllactosamines. Thus, α2-3 sialyl linkages cleave much easier than α2-6 sialyl linkages in MALDI-PSD fragmentation method. These results suggest that the new techniques using PSD fragmentation of MALDI-TOF mass spectrometry enables us to distinguish α2-3 sialyl linkage from α2-6 linkage in sialyl oligosaccharides.

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Reference

  1. Xu G, Suzuki T, Maejima Y, Mizoguchi T, Tsuchiya M, Kiso M, Hasegawa A, Suzuki Y (1995) Glycoconjugate J 12: 156–161.

    Google Scholar 

  2. Cointe D, Leroy Y, Chirat F (1998) Carbohydr Res 311:51–9.

    Google Scholar 

  3. Lemoine J, Strecker G, Leroy Y, Forurnet B (1991) Carbohydr Res 221: 209–17.

    Google Scholar 

  4. Cornish TJ, Cotter RJ (1994) Rapid Commun Mass Spectrom 8: 781–85.

    Google Scholar 

  5. Yamagaki T, Ishizuka Y, Kawabata S, Nakanishi H (1996) Rapid Commun Mass Spectrom 10: 1887–90.

    Google Scholar 

  6. Yamagaki T, Ishizuka Y, Kawabata S, Nakanishi H (1997) Rapid Commun Mass Spectrom 11: 527–31.

    Google Scholar 

  7. Yamagaki T, Mitsuishi Y, Nakanishi H (1998) Rapid Commun Mass Spectrom 12: 307–11.

    Google Scholar 

  8. Yamagaki T, Mitsuishi Y, Nakanishi H (1998) Tetrahedron Lett 39: 4051–54.

    Google Scholar 

  9. Yamagaki T, Mitsuishi Y, Nakanishi H (1998) Biosci Biotechnol Biochem 62: 2470–75.

    Google Scholar 

  10. Papac DI, Wong A, Jones AJS (1996) Anal Chem 68: 3215–23.

    Google Scholar 

  11. Nonami H, Fukui S, Erra-Balsells R (1997) J Mass Spectrom 32: 287–96.

    Google Scholar 

  12. Nonami H, Tanaka K, Fukuyama, Erra-Balsells R (1998) Rapid Commun Mass Spectrom 12: 285–96.

    Google Scholar 

  13. Domon B, Costello CE (1988) Glycoconjugate J 5: 397–409.

    Google Scholar 

Download references

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Authors and Affiliations

  1. National Institute of Bioscience & Human-Technology, 1-1, Higashi, Tsukuba, Ibaraki, 305-8566, Japan

    Tohru Yamagaki & Hiroshi Nakanishi

Authors
  1. Tohru Yamagaki
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  2. Hiroshi Nakanishi
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Yamagaki, T., Nakanishi, H. A new technique distinguishing α2-3 sialyl linkage from α2-6 linkage in sialyllactoses and sialyl-N-acetyllactosamines by post-source decay fragmentation method of MALDI-TOF mass spectrometry. Glycoconj J 16, 385–389 (1999). https://doi.org/10.1023/A:1007050909293

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  • DOI: https://doi.org/10.1023/A:1007050909293

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