Abstract
Effects of dopamine on the membrane permeability transition, thioredoxin reductase activity, production of free radicals and oxidation of sulfhydryl groups in brain mitochondria and the Ca2+ uptake by Na+-Ca2+ exchange and sulfhydryl oxidation in brain synaptosomes were examined. The brain mitochondrial swelling and the fall of transmembrane potential were altered by pretreatment of dopamine in a dose dependent manner. Depressive effect of dopamine on mitochondrial swelling was reversed by 10 μg/ml catalase, and 10 mM DMSO. The activities of thioredoxin reductase in intact or disrupted mitochondria were decreased by dopamine (1-100 μM), 25 μM Zn2+ and 50 μM Mn2+. Dopamine-inhibited enzyme activity was reversed by 10 μg/ml SOD and 10 μg/ml catalase. Pretreatment of dopamine decreased Ca2+ transport in synaptosomes, which was restored by 10 μg/ml SOD and 10 mM DMSO. Dopamine (1-100 μM) in the medium containing mitochondria produced superoxide anion and hydrogen peroxide, while its effect on nitrite production was very weak. The oxidation of sulfhydryl groups in mitochondria and synaptosomes were enhanced by dopamine with increasing incubation times. Results suggest that dopamine could modulate membrane permeability in mitochondria and calcium transport at nerve terminals, which may be ascribed to the action of free radicals and the loss of reduced sulfhydryl groups.
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Kim, K.J., Jang, Y.Y., Hang, E.S. et al. Modulation of brain mitochondrial membrane permeability and synaptosomal Ca2+ transport by dopamine oxidation. Mol Cell Biochem 201, 89–98 (1999). https://doi.org/10.1023/A:1007008417342
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DOI: https://doi.org/10.1023/A:1007008417342