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Structure of the O-glycopeptides isolated from bovine milk component PP3

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Abstract

The heat-stable acid-soluble phosphoglycoprotein component PP3 was isolated from the bovine milk proteose peptone fraction by concanavalin A affinity chromatography. Glycopeptides from the ConA-bound fraction corresponding to the component PP3 were obtained by Pronase digestion and were separated by gel filtration into high and low-molecular-mass glycopeptides. In a previous work, we have investigated the structure of the N-glycans from the high-molecular-mass glycopeptides [Girardet et al. (1995) Eur J Biochem 234: 939–46]. Here, we describe the structure of the O-glycans from the low-molecular-mass glycopeptides. By combining methylation analysis, mass spectrometry, 400 MHz 1H-NMR spectroscopy and peptide sequence analysis, we show that the low-molecular-mass fraction contains several neutral glycopeptides. A mixture of the following three glycan structures linked to the Thr86 has been identified: GalNacα1-O-Thr, Gal(β1-3)GalNAcα1-O-Thr and Gal(β1-4)GlcNAc(β1-6)[Galβ1-3)]GalNAcα1-O-Thr. © 1998 Rapid Science Ltd

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Authors and Affiliations

  1. Laboratoire de Chimie Biologique, Unite Mixte de Recherche du Centre National de la Recherche Scientifique n° 111, Universite des Sciences et Technologies de Lille, 59655, Villeneuve d’Ascq Cedex, France

    Bernadette Coddeville, Yves Plancke & Genevieve Spik

  2. Laboratoire des Biosciences de l’Aliment, Unite Associee a l’Institut National de la Recherche Agronomique, Faculte des Sciences, Universite Henri, Poincare-Nancy 1, B.P. 239, 54506, Vandoeuvre-les-Nancy Cedex, France

    Jean-Michel Girardet, Sylvie Campagna & Guy Linden

Authors
  1. Bernadette Coddeville
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  2. Jean-Michel Girardet
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  3. Yves Plancke
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  4. Sylvie Campagna
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  5. Guy Linden
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  6. Genevieve Spik
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Coddeville, B., Girardet, JM., Plancke, Y. et al. Structure of the O-glycopeptides isolated from bovine milk component PP3. Glycoconj J 15, 371–378 (1998). https://doi.org/10.1023/A:1006973802139

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