Skip to main content

Advertisement

SpringerLink
Log in

Curcumin treatment modulates collagen metabolism in isoprot3erenol induced myoxardial necrosis in rats

  • Published:
Molecular and Cellular Biochemistry Aims and scope Submit manuscript

Abstract

This study was carried out to evaluate whether curcumin, a potent antioxidant, had any specific role in the synthesis and degradation of collagen in rat heart with mocardial necrosis, induced by isoproterenol.HCI (ISO). Myocardial necrosis was induced by administration of ISO (30 mg/100 g body weight subcutaneously twice at an interval of 24 h) and studies on collagen metabolism were carried out with curcumin (200 mg/kg) pre-and co-treatment with ISO. The incorporation of 14C-proline into collagen was studied as an index of collagen synthesis. The heart weight /body weight ratio,heart RNA/DNA ratio and protein were found to increase significantly in ISO administered animals. Curcumin pre- and co-treatment with ISO reversed these changes and attenuated the development of cardiac hypertrophy two weeks after the second dose of ISO. Increased fractional synthesis rate and enhanced degradation of newly synthesized collagen were observed in ISO administered animals. Curcumin pre- and co-treatment with ISO was noticed to decrease the degree of degradation of the existing collagen matrix and collagen synthesis, two weeks after the second dose of ISO. The observed effects could be due to free radical scavenging capacity and inhibition of lysosomal enzyme release by curcumin.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Factor SM, Robinson TF: Comparative connective tissue structure function relationships in biologic pumps. Lab Invest 58: 150–156, 1988

    Google Scholar 

  2. Eghbali M, Biumenfeld OO, Seifter S: Localization of types I, III and IV collagen mRNAs in rat heart cells by in situ hybridization. J Mol Cell Cardiol 21: 103–113, 1989

    Google Scholar 

  3. Cannon RO, Butany TN, McManus BM: Early degradation of collagen from acute myocardial necrosis in the rat. Am J Cardiol 52: 390–395, 1983

    Google Scholar 

  4. Takahashi S, Barry AC, Factor SM: Collagen degradation in ischaemic rat hearts. Biochem J 265: 233–241, 1990

    Google Scholar 

  5. Ravichandran LV, Puvanakrishnan R: In vivo labelling studies on the biosynthesis and degradation of collagen in experimental myocardial infarction. Biochem Int 24: 405–414, 1991

    Google Scholar 

  6. Ammon HP, Wahl MA: Pharmacolgy of curcuma longa. Planta Medica 57: 1–7, 1991

    Google Scholar 

  7. Sharma OP: Antioxidant activity of curcumin and related compounds. Biochem Pharmacol 25: 1811–1812, 1976

    Google Scholar 

  8. Srimal RC: Curcumin. Drugs Future 12: 331–333, 1987

    Google Scholar 

  9. Nagabhushan M, Bhide SV: Curcumin as an inhibitor of Cancer. J Am Coll Nutr 11: 192–198, 1992

    Google Scholar 

  10. Lic J, Zhang I-J, Dezube BJ, Crumpacker CS, Pardee AB: Three inhibitors of Type-I human immunodeficiency virus long terminal repeat directed gene expression and virus replication. Proc Natl Acad Sci USA 90: 1839–1842, 1993

    Google Scholar 

  11. Mazumder A, Raghavan K, Weinstein J, Kohn KW, Pommier Y: Inhibition of human immunodeficiency virus Type-I integrase by curcumin. Biochem Pharmacol 8: 1165–1170, 1995

    Google Scholar 

  12. Nirmala C, Puvanakrishnan R: Effect of curcumin on certain lysosomal hydrolases in isoproterenol induced myocardial infarction in rats. Biochem Pharmacol 51: 47–51, 1996

    Google Scholar 

  13. Mays PK, McAnulty RJ, Campa JS, Laurent GJ: Age related changes in collagen synthesis and degradation in rat tissues. Biochem J 276: 307–313, 1991

    Google Scholar 

  14. Rojkind, Gonzalez E: An improved method for determining specific radioactivities of proline-14C and hydroxyprolin-14C in collagen and noncollagenous proteins. Anal Biochem 57: 1–7, 1974

    Google Scholar 

  15. Laurent GJ: Rates of collagen synthesis in lung, skin and muscle obtained in vivo by a simplified method using [3H] proline. Biochem J 206: 535–544, 1982

    Google Scholar 

  16. Laurent GJ, McAnulty RJ, Gibson JS: Changes in collagen synthesis and degradation during skeletal muscle growth. Am J Physiol 249: C352–C355, 1985

    Google Scholar 

  17. Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ: Protein measurement with folin phenol reagent. J Biol Chem 193: 265–275, 1951

    Google Scholar 

  18. Schneider WC: Phosphorus compound in animal tissue. Extraction and estimation of deoxypentose nucleic acid and pentose nucleic acid. J Biol Chem 161: 293–303, 1945

    Google Scholar 

  19. Morkin E, Ashford TP: Myocardial DNA synthesis in experimental cardiac hypertrophy. Am J Physiol 215: 1409–1413, 1968

    Google Scholar 

  20. Nirmala C, Puvanakrishnan R: Protective role of curcumin against isoproterenol induced myocardial infarction in rats. Mol Cell Biochem 159: 85–93, 1996

    Google Scholar 

  21. Judd JT, Wexler BC: Myocardial glycoprotein changes with isoproterenol induced necrosis and repair in the rat. Am J Physiol 226: 597–602, 1974

    Google Scholar 

  22. Collins P, Billings CG, Barer GR, Daly JJ, Jolly A: Quantitation of isoprenaline induced changes in the ventricular myocardium. Cardiovas Res 9:797–808, 1975

    Google Scholar 

  23. Smits JFM, van Krimpen C, Schoemaker RG: Angiotensin II receptor blockade after myocardial infarction in rats: effects on hemodynamics, myocardial DNA synthesis and interstitial collagen content. J Cardiovas Pharmacol 20: 772–778, 1992

    Google Scholar 

  24. Huei-Chen Huang, Tong Rong Jan, Sheau-Fam Yeh: Inhibitory effect of curcumin, an anti-inflammatory agent, on vascular smooth muscle cell proliferation. Eur J Pharmacol 221: 381–384, 1992

    Google Scholar 

  25. Krimpen VC, Smits JFM, Cleutjens JPM: DNA synthesis in the non-infarcted cardiac interstitium after left coronary artery ligation in the rat: effects of captopril. J Mol Cell Cardiol 23: 1245–1253, 1991

    Google Scholar 

  26. Zak R, Kizu A, Bugalsky L: Cardiac hypertrophy, its characteristics as a growth process. Am J Cardiol 44: 941–946, 1979

    Google Scholar 

  27. Morkin E, Kimata S: Replacement of myosin during development of cardiac hypertrophy. Circ Res 3: 50–57, 1974

    Google Scholar 

  28. Schneir ML, Ramamurthy NS, Golub LM: Extensive degradation of recently synthesized collagen in gingiva of normal and streptozotocin-induced diabetic rats. J Dent Res 63: 23–27, 1984

    Google Scholar 

  29. Laurent GJ, McAnulty RJ: Protein metabolism during bleomycin induced pulmonary fibrosis in rabbits. Amer Rev Respir Dis 128: 82–88, 1983

    Google Scholar 

  30. Baum BJ, Moss J, Breul SD, Berg RA, Crystal RG: Effect of cAMP on the intracellular degradation of newly synthesized collagen. J Biol Chem 255: 2843–2847, 1980

    Google Scholar 

  31. Ohta H, Azuma J, Awata N: Mechanism of the protective action of taurine against isoprenaline induced myocardial damage. Cardiovas Res 22: 407–413, 1988

    Google Scholar 

  32. Schimke RT, Katunuma N: Intracellular protein turnover, pp 173–186. Academic Press, New York, 1975

    Google Scholar 

  33. Berg RA, Schwartz ML, Crystal RG: Regulation of the production of secretory proteins: Intracellular degradation of newly synthesized defective collagen. Proc Natl Acad Sci USA 77: 4746–4750, 1980

    Google Scholar 

  34. Effects of lysosomal cathepsin B, plasmin and kallikrein and spontaneous activation. Biochem J 166: 21–27, 1979

  35. Singal PK, Kapur N, Beamish RE, Dhalla NS: Role of free radicals in catecholamine-induced cardiomyopathy. Can J Physiol Pharmacol 60: 1390 -1397, 1982

    Google Scholar 

  36. Judd JT, Wexler BC: Prolyl hydrolase and collagen metabolism after experimental myocardial infarction. Am J Physiol 228: 212–216, 1975

    Google Scholar 

  37. Geesin JC, Gordon JS, Berg RA: Retinoids affect collagen synthesis through inhibition of ascorbate-induced lipid peroxidation in cultured human dermal fibroblasts. Arch Biochem Biophys 278: 350–355, 1990

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Biotechnology, Central Leather Research Institute, Adyar, Madras, India

    Chandrasekar Nirmala, Selvaraj Anand & Rengarajulu Puvanakrishnan

Authors
  1. Chandrasekar Nirmala
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Selvaraj Anand
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rengarajulu Puvanakrishnan
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Nirmala, C., Anand, S. & Puvanakrishnan, R. Curcumin treatment modulates collagen metabolism in isoprot3erenol induced myoxardial necrosis in rats. Mol Cell Biochem 197, 31–37 (1999). https://doi.org/10.1023/A:1006960106929

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1006960106929

Search

Navigation